Difference between revisions of "Part:BBa K3629001"

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===Usage and Biology===
 
===Usage and Biology===
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This is the natural promoter found in the wild-type <i>Yarrowia lipolytica</i> for the Translation Elongation Factor 1 (TEF1) gene. TEF promoter is among the strongest constitutive promoters found in <i>Yarrowia lipolytica.</i> Inclusion of the first intron of the TEF1 gene in the promoter sequence has shown increase the expression levels (1). It can be placed immediately upstream of a coding sequence for Y. lipolytica.
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This is the natural promoter found in the wild-type <i>Yarrowia lipolytica</i> for the Translation Elongation Factor 1 (TEF1) gene. TEF promoter is among the strongest constitutive promoters found in <i>Yarrowia lipolytica.</i> Inclusion of the first intron of the TEF1 gene in the promoter sequence has shown increase the expression levels (1). It can be placed immediately upstream of a coding sequence for <i>Y. lipolytica.</i>
  
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This promoter was used in the following expression constructs in [https://2020.igem.org/Team:Calgary/Part_Collection our collection:]
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[https://parts.igem.org/Part:BBa_K3529012 BBa_K3529012]= <i>T. reesei</i> CBHII expression construct
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[https://parts.igem.org/Part:BBa_K3529013 BBa_K3529013]= Modified <i>P. funiculosum</i> CBHI expression construct
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[https://parts.igem.org/Part:BBa_K3529014 BBa_K3529014]= <i>N. crassa</i> CBHI expression construct
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[https://parts.igem.org/Part:BBa_K3529016 BBa_K3529016]= Modified <i>T. reesei</i> EGI expression construct
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[https://parts.igem.org/Part:BBa_K3529023 BBa_K3529023]= 2-isopropylmalate synthase (LEU4) overexpression construct
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[https://parts.igem.org/Part:BBa_K3529024 BBa_K3529024]= Anthranilate synthase component 1 (TRP2) overexpression construct
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[https://parts.igem.org/Part:BBa_K3529025 BBa_K3529025]= mCherry expression construct
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[https://parts.igem.org/Part:BBa_K3529026 BBa_K3529026]= mCitrine expression construct
  
 
===Sequence and Features===
 
===Sequence and Features===

Revision as of 21:55, 26 October 2020


Yarrowia lipolytica TEF1 promoter with an intron

Promoter from the Yarrowia lipolytica Translation Elongation Factor 1 (TEF1) gene with the first intron.

Usage and Biology

This is the natural promoter found in the wild-type Yarrowia lipolytica for the Translation Elongation Factor 1 (TEF1) gene. TEF promoter is among the strongest constitutive promoters found in Yarrowia lipolytica. Inclusion of the first intron of the TEF1 gene in the promoter sequence has shown increase the expression levels (1). It can be placed immediately upstream of a coding sequence for Y. lipolytica.


This promoter was used in the following expression constructs in our collection:

BBa_K3529012= T. reesei CBHII expression construct BBa_K3529013= Modified P. funiculosum CBHI expression construct BBa_K3529014= N. crassa CBHI expression construct BBa_K3529016= Modified T. reesei EGI expression construct BBa_K3529023= 2-isopropylmalate synthase (LEU4) overexpression construct BBa_K3529024= Anthranilate synthase component 1 (TRP2) overexpression construct BBa_K3529025= mCherry expression construct BBa_K3529026= mCitrine expression construct

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


The wild-type sequence of this promoter contains BsaI, PstI, and SpeI restriction sites in the promoter section before the intron sequence. Therefore, single-nucleotides changes that match the ones in the functional BBa_K2983050 promoter were made to remove these sites.

References

1. Blazeck, J., Liu, L., Redden, H., & Alper, H. (2011). Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach. Applied and environmental microbiology, 77(22), 7905–7914. https://doi.org/10.1128/AEM.05763-11

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