Difference between revisions of "Part:BBa K3629001"
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===Sequence and Features=== | ===Sequence and Features=== | ||
<partinfo>BBa_K3629001 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3629001 SequenceAndFeatures</partinfo> | ||
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| + | The wild-type sequence of this promoter contains BsaI, PstI, and SpeI restriction sites in the promoter section before the intron sequence. Therefore, single-nucleotides changes that match the ones in the functional [https://parts.igem.org/Part:BBa_K2983050 BBa_K2983050] promoter were made to remove these sites. | ||
===References=== | ===References=== | ||
Revision as of 21:51, 26 October 2020
Yarrowia lipolytica TEF1 promoter with an intron
Promoter from the Yarrowia lipolytica Translation Elongation Factor 1 (TEF1) gene with the first intron.
Usage and Biology
This is the natural promoter found in the wild-type Yarrowia lipolytica for the Translation Elongation Factor 1 (TEF1) gene. TEF promoter is among the strongest constitutive promoters found in Yarrowia lipolytica. Inclusion of the first intron of the TEF1 gene in the promoter sequence has shown increase the expression levels (1). It can be placed immediately upstream of a coding sequence for Y. lipolytica.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
The wild-type sequence of this promoter contains BsaI, PstI, and SpeI restriction sites in the promoter section before the intron sequence. Therefore, single-nucleotides changes that match the ones in the functional BBa_K2983050 promoter were made to remove these sites.
References
1. Blazeck, J., Liu, L., Redden, H., & Alper, H. (2011). Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach. Applied and environmental microbiology, 77(22), 7905–7914. https://doi.org/10.1128/AEM.05763-11
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