Difference between revisions of "Part:BBa K3506050"
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| − | A small guide RNA (sgRNA) component created by fusing an approximately 20 nt target-sequence RNA (crRNA) to a tailored bacterial tracrRNA. We design the sgRNA targeted on ADE2 to specifically knock out this gene in Cryptococcus neoformans. | + | A small guide RNA (sgRNA) component created by fusing an approximately 20 nt target-sequence RNA (crRNA) to a tailored bacterial tracrRNA. We design the sgRNA targeted on <i>ADE2</i> to specifically knock out this gene in <i>Cryptococcus neoformans</i>. |
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Revision as of 21:48, 26 October 2020
gRNA targets ADE2 gene
A small guide RNA (sgRNA) component created by fusing an approximately 20 nt target-sequence RNA (crRNA) to a tailored bacterial tracrRNA. We design the sgRNA targeted on ADE2 to specifically knock out this gene in Cryptococcus neoformans.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]