Difference between revisions of "Part:BBa K3331000"
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===Characterization===
 
===Characterization===
−
We cloned and confirmed the target fragment by PCR. The length of the E.coli galU fragment is 981bp. The following results showed the successful result.
+
We cloned and confirmed the target fragment by PCR. The length of the B.S galU fragment is 951bp. The following results showed the successful result.
 
<div>[[File:T--XJTU-China--Bggel.png |700px|thumb|center|<b>Figure 1:</b>PCR confirmation of the target fragment.]]</div>
 
<div>[[File:T--XJTU-China--Bggel.png |700px|thumb|center|<b>Figure 1:</b>PCR confirmation of the target fragment.]]</div>

Revision as of 21:44, 26 October 2020


B.S. galU

UTP-glucose-1-phosphate uridylyltransferase (GalU) carries out a key step in the generation of UDP-D-glucuronate.This part catalyzes the addition of UTP to α-D-glucose 1-phosphate to yield UDP-D-glucose

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

We cloned and confirmed the target fragment by PCR. The length of the B.S galU fragment is 951bp. The following results showed the successful result.

Figure 1:PCR confirmation of the target fragment.