Difference between revisions of "Part:BBa K3411010"

Revision as of 20:42, 26 October 2020

NarP Gene

NarP is a part of the Nar Operon which detects both nitrate and nitrite. Our system is modeled after E.coli’s natural nitrite sensor. Similar to NarL system, the promoter BBa_J23106, a constitutive Anderson Promoter, produces TetR, which represses the PtetO promoter, producing NarP. The NarP gene is phosphorylated in the presence of both nitrate and nitrite. Therefore, when NarP eventually phosphorylates PdcuS, which is further explained in the NarP composite Part:BBa_K3411020, it is measuring the levels of both nitrate and nitrite. By using the corrected difference our NarP and NarL composite parts, we are able to evaluate the levels of Nitrite. This specific part is the isolated NarP gene.

Lambert iGEM plans on further characterizing our part in the coming year as a part of our two year project, AgroSense.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3411010 short</partinfo>
-NarP, which detects both nitrate and nitrite. By calculating the corrected difference, our system would have concentrations for both nutrients. Lambert iGEM’s novel NarP system BBa_K3411020 is also modeled after E.coli’s natural nitrite sensor. Similarly to NarL, the promoter BBa_J23106, a constitutive Anderson Promoter, produces TetR, which represses the PtetO promoter, producing NarP. By regulating the amount of aTc, the molecule that inhibits TetR, our lab can control NarP’s expression, the product of the PtetO promoter. NarQ, the native membrane-bound protein, senses the amount of nitrate and/or nitrite in the cell: In the presence of nitrite and/or nitrate, NarQ will phosphorylate NarP, activating it, but if there is no nitrite and/or nitrate present, NarQ will not phosphorylate NarP. If NarP is phosphorylated, it will activate the nirB promoter, producing GFP. Lambert iGEM plans to correlate GFP levels to nitrite through mathematical modeling.
+NarP is a part of the Nar Operon which detects both nitrate and nitrite. Our system is modeled after E.coli’s natural nitrite sensor. Similar to NarL system, the promoter BBa_J23106, a constitutive Anderson Promoter, produces TetR, which represses the PtetO promoter, producing NarP. The NarP gene is phosphorylated in the presence of both nitrate and nitrite. Therefore, when NarP eventually phosphorylates PdcuS, which is further explained in the NarP composite Part:BBa_K3411020, it is measuring the levels of both nitrate and nitrite. By using the corrected difference our NarP and NarL composite parts, we are able to evaluate the levels of Nitrite. This specific part is the isolated NarP gene.
+Lambert iGEM plans on further characterizing our part in the coming year as a part of our two year project, AgroSense.
 <!-- Add more about the biology of this part here