Difference between revisions of "Part:BBa K3504006"

 
 
(7 intermediate revisions by 2 users not shown)
Line 2: Line 2:
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K3504006 short</partinfo>
 
<partinfo>BBa_K3504006 short</partinfo>
 +
<p style="color:red">NOTICE: Parts  in our range for this season have been created as a part of our Phase I design of our project. These parts HAVE NOT been tested or characterized in the lab due to COVID-19-related precautionary measures. We have enriched our new parts pages with data from literature and results from our modeling and simulations. If you are intending on using this part or others in our range, please keep in mind these limitations and update these parts with data from your experimentation. Feel free to reach us at: igem.afcm@gmail.com for further inquiries.</p><br/>
 +
==Part Description==
  
−
Blank
+
MiRNA FF6 is a micro RNA that has the ability to repress transcription. MiRNA FF6 is one of microRNAs that were constructed according to the pPRIME approach with stem-loops of functional sequence cloned into miR-30 backbone and fused into 3’-UTR of CMV-driven neomycin resistance gene. Their names are identical to their parent siRNAmolecules, i.e., FF3, FF4, FF5 and FF6
 +
 +
==Usage==
 +
Genetically encoded miRNA sensors (which sense a solitary miRNA binding) and cell classifiers (which sense different miRNA inputs at the same time) can give data about infection state, activate reactions in cells explicitly communicating either a healthy or diseased miRNA profile. Through analyzing and studying multiple synthetic miRNAs and testing them, we found that miRNA FF6 shows high potency in translational silencing and significant cross-talk in the tumor micro-environment, accordingly we modified our OFF-switch to contain miRNA FF6 target site between the triplex and degradation motifs  in our circuit in the 5’ UTR OFF position. Once miRNA FF6 binds to its target site on the mRNA in the 5’ UTR OFF position, this triggers a series of post-transciption modifications that regulate the gene expression and transcription of the used SFV replicon.(1)
  
−
<!-- Add more about the biology of this part here
 
−
===Usage and Biology===
 
  
 +
==References==
 +
1-DiAndreth, B., Wauford, N., Hu, E., Palacios, S., &amp; Weiss, R. (2019, January 01). PERSIST: A programmable RNA regulation platform using CRISPR endoRNases. Retrieved October 26, 2020, from https://www.biorxiv.org/content/10.1101/2019.12.15.867150v1.full
 
<!-- -->
 
<!-- -->
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 19:34, 26 October 2020


MiRNA FF6

NOTICE: Parts in our range for this season have been created as a part of our Phase I design of our project. These parts HAVE NOT been tested or characterized in the lab due to COVID-19-related precautionary measures. We have enriched our new parts pages with data from literature and results from our modeling and simulations. If you are intending on using this part or others in our range, please keep in mind these limitations and update these parts with data from your experimentation. Feel free to reach us at: igem.afcm@gmail.com for further inquiries.


Part Description

MiRNA FF6 is a micro RNA that has the ability to repress transcription. MiRNA FF6 is one of microRNAs that were constructed according to the pPRIME approach with stem-loops of functional sequence cloned into miR-30 backbone and fused into 3’-UTR of CMV-driven neomycin resistance gene. Their names are identical to their parent siRNAmolecules, i.e., FF3, FF4, FF5 and FF6

Usage

Genetically encoded miRNA sensors (which sense a solitary miRNA binding) and cell classifiers (which sense different miRNA inputs at the same time) can give data about infection state, activate reactions in cells explicitly communicating either a healthy or diseased miRNA profile. Through analyzing and studying multiple synthetic miRNAs and testing them, we found that miRNA FF6 shows high potency in translational silencing and significant cross-talk in the tumor micro-environment, accordingly we modified our OFF-switch to contain miRNA FF6 target site between the triplex and degradation motifs in our circuit in the 5’ UTR OFF position. Once miRNA FF6 binds to its target site on the mRNA in the 5’ UTR OFF position, this triggers a series of post-transciption modifications that regulate the gene expression and transcription of the used SFV replicon.(1)


References

1-DiAndreth, B., Wauford, N., Hu, E., Palacios, S., & Weiss, R. (2019, January 01). PERSIST: A programmable RNA regulation platform using CRISPR endoRNases. Retrieved October 26, 2020, from https://www.biorxiv.org/content/10.1101/2019.12.15.867150v1.full Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]