Difference between revisions of "Part:BBa K3482043"

 
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Azurin protein under a pTet01 promoter
  
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===Usage and Biology===
 
===Usage and Biology===
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Azurin is a blue copper-binding redox protein originally found in the periplasmic membrane of Pseudomonas aeruginosa. It has recently been discovered to have an anticancer effect by targeting the well-known p53 apoptosis pathway and by preferentially entering cancerous cells.
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We used this part for protein expression in a sponge plasmid for oscillatory expression with a repressilator
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Latest revision as of 17:17, 26 October 2020


azurin (UNIL) under pTetO1

Azurin protein under a pTet01 promoter

Usage and Biology

Azurin is a blue copper-binding redox protein originally found in the periplasmic membrane of Pseudomonas aeruginosa. It has recently been discovered to have an anticancer effect by targeting the well-known p53 apoptosis pathway and by preferentially entering cancerous cells.

We used this part for protein expression in a sponge plasmid for oscillatory expression with a repressilator


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 55
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 55
    Illegal NheI site found at 336
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 55
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 55
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 55
  • 1000
    COMPATIBLE WITH RFC[1000]