Difference between revisions of "Part:BBa K3482042"
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<partinfo>BBa_K3482042 short</partinfo>
 
<partinfo>BBa_K3482042 short</partinfo>
  
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The CcdA protein provides immunity against the CcdB toxin by strongly complexing with it (CcdA-CcdB) and therefore prenventing the CcdB toxin to interact with the DNA gyrase.
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Azurin protein fused with NSP4 secretion signial peptide and a 8His secretion tag.
  
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This part is used with an aTc inducible promoter to form a new part <html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K3482020"> BBa_K3482020 </a></html>.
 
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===Usage and Biology===
 
===Usage and Biology===
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Azurin is a blue copper-binding redox protein originally found in the periplasmic membrane of Pseudomonas aeruginosa. It has recently been discovered to have an anticancer effect by targeting the well-known p53 apoptosis pathway and by preferentially entering cancerous cells.
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We used this part with a tag for protein expression and purification
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Latest revision as of 17:16, 26 October 2020


azurin with NSP4 secretion and 8His tag

Azurin protein fused with NSP4 secretion signial peptide and a 8His secretion tag.

Usage and Biology

Azurin is a blue copper-binding redox protein originally found in the periplasmic membrane of Pseudomonas aeruginosa. It has recently been discovered to have an anticancer effect by targeting the well-known p53 apoptosis pathway and by preferentially entering cancerous cells.

We used this part with a tag for protein expression and purification


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 48
  • 1000
    COMPATIBLE WITH RFC[1000]