Difference between revisions of "Part:BBa I15010"

 
 
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Chimeric Cph1 light receptor/EnvZ protein.   
 
Chimeric Cph1 light receptor/EnvZ protein.   
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===Usage and Biology===
 
===Usage and Biology===
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Requires PCB biosynthetic genes (BBa_15008 and BBa_15009) for chromophore formation.  The light responsive domain (Cph1) has maximal response to light near 660nm.  Exposure to red light inhibits the activity of the EnvZ histidine kinase domain.  When active (in the dark) EnvZ phosphorylates endogenous OmpR, a transcription factor which activates transcription from the OmpC promoter and represses transcription from the OmpF promoter.  Must be used in E.coli deficient in natural EnvZ. 
 
  
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Some user-experiences and characterization experiments related to the bacterial photography system are documented here [[http://openwetware.org/wiki/Talk:BE.109:Systems_engineering/RT-PCR_data_analysis]]. In general, experiments were conducted by students in BE.109, spring 2006, and involved variation of the strain's growth conditions or light exposure. The consequences of each experimental variation were followed with b-gal assay and RT-PCR.  
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Requires PCB biosynthetic genes (BBa_I15008 and BBa_I15009) for chromophore formation.  The light responsive domain (Cph1) has maximal response to light near 660nm.  Exposure to red light inhibits the activity of the EnvZ histidine kinase domain.  When active (in the dark) EnvZ phosphorylates endogenous OmpR, a transcription factor which activates transcription from the OmpC promoter and represses transcription from the OmpF promoter.  Must be used in E.coli deficient in natural EnvZ. 
 +
 
 +
Some user-experiences and characterization experiments related to the bacterial photography system are documented here [http://openwetware.org/wiki/Talk:BE.109:Systems_engineering/RT-PCR_data_analysis]. In general, experiments were conducted by students in BE.109, spring 2006, and involved variation of the strain's growth conditions or light exposure. The consequences of each experimental variation were followed with b-gal assay and RT-PCR.  
  
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_I15010 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_I15010 SequenceAndFeatures</partinfo>
  
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<!-- Uncomment this to enable Functional Parameter display
 
===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_I15010 parameters</partinfo>
 
<partinfo>BBa_I15010 parameters</partinfo>
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Latest revision as of 17:12, 26 October 2020


cph8 (Cph1/EnvZ fusion)

Chimeric Cph1 light receptor/EnvZ protein.


Usage and Biology

Requires PCB biosynthetic genes (BBa_I15008 and BBa_I15009) for chromophore formation. The light responsive domain (Cph1) has maximal response to light near 660nm. Exposure to red light inhibits the activity of the EnvZ histidine kinase domain. When active (in the dark) EnvZ phosphorylates endogenous OmpR, a transcription factor which activates transcription from the OmpC promoter and represses transcription from the OmpF promoter. Must be used in E.coli deficient in natural EnvZ.

Some user-experiences and characterization experiments related to the bacterial photography system are documented here [http://openwetware.org/wiki/Talk:BE.109:Systems_engineering/RT-PCR_data_analysis]. In general, experiments were conducted by students in BE.109, spring 2006, and involved variation of the strain's growth conditions or light exposure. The consequences of each experimental variation were followed with b-gal assay and RT-PCR.

Sequence and Features


Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2257
    Illegal XhoI site found at 364
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]