Difference between revisions of "Part:BBa K864400:Experience"

Revision as of 16:51, 26 October 2020

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K864400

Team INSA Lyon 2016

Author: Gonthier Mathilde

Summary: The INSA Lyon 2016 team used this part in order to characterize it. A GFP or a CFP coding genes were set after this promotor, by measuring the fluorescence of the expressions cultures having these constructs the team was able to gather some informations about the behavior of this promotor. For further informations you may consult both BBa_K1934040 and BBa_K1934050 registry pages.

Team GreatBay_SZ 2020

We have used pTac to express the key protein of our project: Geobacter sulfurreducens' pilin-based electrically conductive protein nanowires (e-PNs). The gene circuit includes this tac promoter which promotes the pilin gene(pilA) to express protein monomers; the monomers are later assembled into protein nanowires by the TypeIV pilin assembly system, which is constructed from the gene cluster at the downstream of the pilin gene(figure 1).

Figure 1: A Construction of BIOT gene circuit

The e-PN can be successfully expressed by express this part under the control of this pTac, and the result was verified by Western Blot(figure 2).

Figure 2: Western Blot of the cell lysis of BIOT and BIOT-His(express e-PN and e-PN-His respectively); Antibody: Anti-His tag; e-PN-His: 7.7 kDa.

User Reviews

•

mbecich

This part function as described by our team, it's an efficient promotor. But notice that it tends to leak after a few hours.

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 <h3>Team GreatBay_SZ 2020</h3>
-<p>We have used pTac to express the key protein of our project: <i>Geobacter sulfurreducens<i>' pilin-based electrically conductive protein nanowires (e-PNs). The gene circuit includes this tac promoter which promotes the pilin gene(<i>pilA</i>) to express protein monomers; the monomers are later assembled into protein nanowires by the TypeIV pilin assembly system, which is constructed from the gene cluster at the downstream of the pilin gene(figure 1).  </p>
+<p>We have used pTac to express the key protein of our project: <i>Geobacter sulfurreducens</i>' pilin-based electrically conductive protein nanowires (e-PNs). The gene circuit includes this tac promoter which promotes the pilin gene(<i>pilA</i>) to express protein monomers; the monomers are later assembled into protein nanowires by the TypeIV pilin assembly system, which is constructed from the gene cluster at the downstream of the pilin gene(figure 1).  </p>
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 <img src="https://2020.igem.org/wiki/images/6/64/T--GreatBay_SZ--circuit2.png" alt="" width="700">
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 <img src="https://2020.igem.org/wiki/images/4/4e/T--GreatBay_SZ--western.png" alt="" width="400">
 <h6 style="text-align:center">Figure 2: Western Blot of the cell lysis of BIOT and BIOT-His(express e-PN and e-PN-His respectively); Antibody: Anti-His tag; e-PN-His: 7.7 kDa.</h6>  </div>
+</html>
 ===User Reviews===
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