Difference between revisions of "Part:BBa K3651035"

Latest revision as of 16:31, 26 October 2020

Iron sensitive promoter pfur23 upstream of mCherry.

This transcription unit includes an iron sensitive promoter with two Ferric Uptake Regulator (FUR) Binding site (BBa_K3651023) upstream a ribosome binding site (BBa_J61100), red fluorescence protein (BBa_K3651025), and terminator (BBa_B0010). Altogether, this is an iron sensor. When iron concentration increases (in the presence of FUR protein), red fluorescence decreases. The FUR Protein in presence of iron will block the transcription because it will bind to its FUR Box.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3651035 short</partinfo>
-This transcription unit includes an iron sensitive promoter with three Ferric Uptake Regulator (FUR) Binding site (BBa_K3651023) upstream a ribosome binding site (BBa_J61100), red fluorescence protein (BBa_K3651025), and terminator (BBa_B0010). Altogether, this is an iron sensor. When iron concentration increases (in the presence of FUR protein), red fluorescence decreases. The FUR Protein in presence of iron will block the transcription because it will bind to its FUR Box.
+This transcription unit includes an iron sensitive promoter with two Ferric Uptake Regulator (FUR) Binding site (BBa_K3651023) upstream a ribosome binding site (BBa_J61100), red fluorescence protein (BBa_K3651025), and terminator (BBa_B0010). Altogether, this is an iron sensor. When iron concentration increases (in the presence of FUR protein), red fluorescence decreases. The FUR Protein in presence of iron will block the transcription because it will bind to its FUR Box.
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