Difference between revisions of "Part:BBa K3450007"
 
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<partinfo>BBa_K3450007 short</partinfo>
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The N-terminus of β-intimin, termed Neae, is a stable transmembrane base, facilitating the display of proteins fused to its C-terminus on E. coli’s surface. It is derived from the cell surface adhesin β-intimin from enterohemorrhagic E. coli (EHEC), responsible for binding to the Type III Secretion System effector Tir (Translocated Intimin Receptor), displayed on the apical surface of intestinal epithelial cells.
 
The N-terminus of β-intimin, termed Neae, is a stable transmembrane base, facilitating the display of proteins fused to its C-terminus on E. coli’s surface. It is derived from the cell surface adhesin β-intimin from enterohemorrhagic E. coli (EHEC), responsible for binding to the Type III Secretion System effector Tir (Translocated Intimin Receptor), displayed on the apical surface of intestinal epithelial cells.
  
 
When used to display proteins, β-intimin exhibited the ability to serve as a non-toxigenic base that did not leave its chassis susceptible to membrane damage. It has been used to display single-domain antibodies (sdAbs) on E. coli’s surface, directing the adhesion of its chassis to in vivo tumour cells bearing target antigens.
 
When used to display proteins, β-intimin exhibited the ability to serve as a non-toxigenic base that did not leave its chassis susceptible to membrane damage. It has been used to display single-domain antibodies (sdAbs) on E. coli’s surface, directing the adhesion of its chassis to in vivo tumour cells bearing target antigens.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3450007 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K3450007 parameters</partinfo>
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Latest revision as of 15:19, 26 October 2020

β-intimin Neae: Intimin is an important virulence factor of EHEC and mediates intimate adhesion


The N-terminus of β-intimin, termed Neae, is a stable transmembrane base, facilitating the display of proteins fused to its C-terminus on E. coli’s surface. It is derived from the cell surface adhesin β-intimin from enterohemorrhagic E. coli (EHEC), responsible for binding to the Type III Secretion System effector Tir (Translocated Intimin Receptor), displayed on the apical surface of intestinal epithelial cells.

When used to display proteins, β-intimin exhibited the ability to serve as a non-toxigenic base that did not leave its chassis susceptible to membrane damage. It has been used to display single-domain antibodies (sdAbs) on E. coli’s surface, directing the adhesion of its chassis to in vivo tumour cells bearing target antigens.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 529
    Illegal PstI site found at 607
    Illegal PstI site found at 619
    Illegal PstI site found at 649
    Illegal PstI site found at 1096
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 529
    Illegal PstI site found at 607
    Illegal PstI site found at 619
    Illegal PstI site found at 649
    Illegal PstI site found at 1096
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 529
    Illegal PstI site found at 607
    Illegal PstI site found at 619
    Illegal PstI site found at 649
    Illegal PstI site found at 1096
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 529
    Illegal PstI site found at 607
    Illegal PstI site found at 619
    Illegal PstI site found at 649
    Illegal PstI site found at 1096
    Illegal NgoMIV site found at 801
    Illegal NgoMIV site found at 1542
  • 1000
    COMPATIBLE WITH RFC[1000]