Difference between revisions of "Part:BBa K3505024"

Revision as of 15:04, 26 October 2020

B-arrestin:TEV Protease GB compatible with B2-B5

long

Usage and Biology

Design Notes

The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is presents in pUPD2 and has overhangs compatible for Golden Braid cloning. The CDS has position B3-B5.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal SpeI site found at 1611
Illegal PstI site found at 303
Illegal PstI site found at 390
12
INCOMPATIBLE WITH RFC[12]
Illegal SpeI site found at 1611
Illegal PstI site found at 303
Illegal PstI site found at 390
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 203
Illegal XhoI site found at 600
Illegal XhoI site found at 994
23
INCOMPATIBLE WITH RFC[23]
Illegal SpeI site found at 1611
Illegal PstI site found at 303
Illegal PstI site found at 390
25
INCOMPATIBLE WITH RFC[25]
Illegal SpeI site found at 1611
Illegal PstI site found at 303
Illegal PstI site found at 390
Illegal AgeI site found at 586
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 1545
Illegal SapI.rc site found at 1893

@@ Line 5: / Line 5: @@
 long
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
-<!-- -->
-<span class='h3bb'>Sequence and Features</span>
-<partinfo>BBa_K3505024 SequenceAndFeatures</partinfo>
-<!-- Uncomment this to enable Functional Parameter display
+===Design Notes===
-===Functional Parameters===
+The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is presents in pUPD2 and has overhangs compatible for Golden Braid cloning.
-<partinfo>BBa_K3505024 parameters</partinfo>
+The CDS has position B3-B5.
-<!-- -->
+===Sequence and Features===
+<partinfo>BBa_K3505024 SequenceAndFeatures</partinfo>