Difference between revisions of "Part:BBa K3693004:Design"

 
 
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__NOTOC__
 
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<partinfo>BBa_K3693003 short</partinfo>
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<partinfo>BBa_K3693004 short</partinfo>
  
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<partinfo>BBa_K3693003 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K3693004 SequenceAndFeatures</partinfo>
  
  
 
===Design Notes===
 
===Design Notes===
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There are two considerations that we have during the detailed design of this sequence. On the one hand, we acknowledged the differences between humans yeasts,doing codon optimization to this sequence in order to increase protein expression. On the other hand, we put His-tag to the sequence, which indeed helps purify proteins.
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We add a his tag between secretion signal and gene for purification.
  
  
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===Source===
 
===Source===
  
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It comes from the result of PCR of genomes.
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The alpha-secretion signal is from pichia pastoris. His-tag is we designed and added artificially. The original sequence of CSN3 is from Homo sapiens but we did codon optimization.
  
 
===References===
 
===References===

Latest revision as of 14:52, 26 October 2020


For secreting the κ-casein outside yeast


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We add a his tag between secretion signal and gene for purification.


Source

The alpha-secretion signal is from pichia pastoris. His-tag is we designed and added artificially. The original sequence of CSN3 is from Homo sapiens but we did codon optimization.

References