Difference between revisions of "Part:BBa K3693004"

 
 
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<partinfo>BBa_K3693003 short</partinfo>
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<partinfo>BBa_K3693004 short</partinfo>
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This gene encodes alpha-lactalbumin, a principal protein of milk. Alpha-lactalbumin forms the regulatory subunit of the lactose synthase (LS) heterodimer and beta 1,4-galactosyltransferase (beta4Gal-T1) forms the catalytic component. Together, these proteins enable LS to produce lactose by transfering galactose moieties to glucose. As a monomer, alpha-lactalbumin strongly binds calcium and zinc ions and may possess bactericidal or antitumor activity. A folding variant of alpha-lactalbumin, called HAMLET, likely induces apoptosis in tumor and immature cells.
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The composite part consists of a secretion signal for pichia pastoris that can trigger the expression of the protein exerted in, and followed by a his tag for purification, then the sequence of CSN3 we exerted in. This sequence enables the yeast to produce kappa-casein and secrete it outside.
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3693003 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K3693004 SequenceAndFeatures</partinfo>
  
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K3693003 parameters</partinfo>
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<partinfo>BBa_K3693004 parameters</partinfo>
 
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Latest revision as of 14:48, 26 October 2020


For secreting the κ-casein outside yeast

The composite part consists of a secretion signal for pichia pastoris that can trigger the expression of the protein exerted in, and followed by a his tag for purification, then the sequence of CSN3 we exerted in. This sequence enables the yeast to produce kappa-casein and secrete it outside.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]