Difference between revisions of "Part:BBa K3693002:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
There are two considerations that we have during the detailed design of this sequence. On the one hand, we acknowledged the differences between humans yeasts,doing codon optimization to this sequence in order to increase protein expression. On the other hand, we put His-tag to the sequence, which indeed helps purify proteins.
+
There are two considerations that we have during the detailed design of this sequence. On the one hand, we acknowledged the differences between humans yeasts,doing codon optimization to this sequence in order to increase protein expression. On the other hand, we delete the stop codon in order to add tag or construct chimera easily.
−
 
+
−
 
+
  
 
===Source===
 
===Source===
  
−
atgaaatcttttttgttggttgttaacgctttggctttgaccttgccatttttggctgttgaagttcaaaaccaaaaacaaccagcttgtcacgaaaacgacgaaagacctttttaccaaaagactgctccatacgttccaatgtattacgttccaaactcttacccttactatggtactaatttgtaccagagaagaccagctatcgctattaacaacccatacgttcctagaacctactacgctaacccagctgttgttagaccacatgctcaaattcctcaaagacagtatttgccaaactcccacccaccaaccgttgttagattgccaaacttgcacccatccttcattgctattccaccaaaaaagattcaagataagattattattccaactattaacactattgctactgttgaaccaactccagctccagctactgaaccaactgttgattctgttgttactccagaagccttttctgaatctattattacttctactccagaaactactactgttgctgttactccaccaactgct
+
The original source is from Homo sapiens, but we did codon optimization.
  
 
===References===
 
===References===

Latest revision as of 14:32, 26 October 2020


coding for κ-casein in Homo sapiens


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There are two considerations that we have during the detailed design of this sequence. On the one hand, we acknowledged the differences between humans yeasts,doing codon optimization to this sequence in order to increase protein expression. On the other hand, we delete the stop codon in order to add tag or construct chimera easily.

Source

The original source is from Homo sapiens, but we did codon optimization.

References