Difference between revisions of "Part:BBa K3606100"
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<partinfo>BBa_K3606100 short</partinfo> | <partinfo>BBa_K3606100 short</partinfo> | ||
− | + | J23106-RBS-luxI-B0015-J23106-RBS-luxR is a composite part constructed to correlate the quorum-sensing module and the calcium absorption module of our project. | |
− | <!-- | + | <!-- --> |
===Usage and Biology=== | ===Usage and Biology=== | ||
+ | <p>The promoter luxpR is activated by a complex comprised of a small molecule called AHL(acyl-homoserine lactone) and a constitutively expressed protein luxR which can bind AHL. LuxI encodes a synthase that converts SAM into AHL.</p> | ||
+ | <p>When the population of our bacteria is low,the amount of the complex is not enough, thus the luxpR promoter cannot initiate expression of downstream genes.</p> | ||
+ | <p>When the population of bacteria reaches a certain level, the AHL-luxR complex triggers the luxpR promoter, so that our engineered probiotics will express and secrete CaAP and play a role in facilitating calcium absorption.</p> | ||
+ | <p>See more information about luxpR-CaAP on BBa_K3606044.</p> | ||
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− | < | + | <h4>Sequence and Features</h4> |
<partinfo>BBa_K3606100 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3606100 SequenceAndFeatures</partinfo> | ||
Latest revision as of 14:13, 26 October 2020
J23106-RBS-luxI-B0015-J23106-RBS-luxR
J23106-RBS-luxI-B0015-J23106-RBS-luxR is a composite part constructed to correlate the quorum-sensing module and the calcium absorption module of our project.
Usage and Biology
The promoter luxpR is activated by a complex comprised of a small molecule called AHL(acyl-homoserine lactone) and a constitutively expressed protein luxR which can bind AHL. LuxI encodes a synthase that converts SAM into AHL.
When the population of our bacteria is low,the amount of the complex is not enough, thus the luxpR promoter cannot initiate expression of downstream genes.
When the population of bacteria reaches a certain level, the AHL-luxR complex triggers the luxpR promoter, so that our engineered probiotics will express and secrete CaAP and play a role in facilitating calcium absorption.
See more information about luxpR-CaAP on BBa_K3606044.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal suffix found in sequence at 701
Illegal EcoRI site found at 1074 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1074
Illegal NheI site found at 11
Illegal NheI site found at 34
Illegal NheI site found at 1096
Illegal NheI site found at 1119
Illegal SpeI site found at 702
Illegal PstI site found at 716
Illegal NotI site found at 709 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1074
Illegal BglII site found at 694 - 23INCOMPATIBLE WITH RFC[23]Illegal suffix found in sequence at 702
Illegal EcoRI site found at 1074 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1074
Illegal SpeI site found at 702
Illegal PstI site found at 716 - 1000COMPATIBLE WITH RFC[1000]