Difference between revisions of "Part:BBa K3606100"
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<partinfo>BBa_K3606100 short</partinfo>
 
<partinfo>BBa_K3606100 short</partinfo>
  
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J23106-RBS-luxI-B0015-luxR
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J23106-RBS-luxI-B0015-J23106-RBS-luxR is a composite part constructed to correlate the quorum-sensing module and the calcium absorption module of our project.
  
 
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===Usage and Biology===
 
===Usage and Biology===
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The promoter luxpR is activated by a complex comprised of a small molecule called AHL(acyl-homoserine lactone) and a constitutively expressed protein luxR which can bind AHL. LuxI encodes a synthase that converts SAM into AHL. 
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When the population of our bacteria is low,the amount of the complex is not enough, thus the luxpR promoter cannot initiate expression of downstream genes.
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When the population of bacteria reaches a certain level, the AHL-luxR complex triggers the luxpR promoter, so that our engineered probiotics will express and secrete CaAP and play a role in facilitating calcium absorption.
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See more information about luxpR-CaAP  on BBa_K3606044.
  
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luxpR_CaAP is a composite part constructed to correlate the quorum-sensing module and the calcium absorption module of our project.The promoter luxpR is activated by the heterodimer of luxI and luxR. When the population of our bacteria is low,the amount of the heterodimer is not enough, thus the luxpR promoter cannot initiate expression of downstream genes.
 
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When the population of bacteria reaches a certain level, the heterodimer triggers the luxpR promoter, so that our engineered probiotics will express and secrete CaAP and play a role in facilitating calcium absorption.
 
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See more information about luxI-luxR-luxpR regulating system on BBa_K3606100.
 
 
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<h4>Sequence and Features</h4>
 
<h4>Sequence and Features</h4>

Revision as of 14:11, 26 October 2020


J23106-RBS-luxI-B0015-J23106-RBS-luxR

J23106-RBS-luxI-B0015-J23106-RBS-luxR is a composite part constructed to correlate the quorum-sensing module and the calcium absorption module of our project.

Usage and Biology

The promoter luxpR is activated by a complex comprised of a small molecule called AHL(acyl-homoserine lactone) and a constitutively expressed protein luxR which can bind AHL. LuxI encodes a synthase that converts SAM into AHL. When the population of our bacteria is low,the amount of the complex is not enough, thus the luxpR promoter cannot initiate expression of downstream genes. When the population of bacteria reaches a certain level, the AHL-luxR complex triggers the luxpR promoter, so that our engineered probiotics will express and secrete CaAP and play a role in facilitating calcium absorption. See more information about luxpR-CaAP on BBa_K3606044.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 701
    Illegal EcoRI site found at 1074
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1074
    Illegal NheI site found at 11
    Illegal NheI site found at 34
    Illegal NheI site found at 1096
    Illegal NheI site found at 1119
    Illegal SpeI site found at 702
    Illegal PstI site found at 716
    Illegal NotI site found at 709
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1074
    Illegal BglII site found at 694
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 702
    Illegal EcoRI site found at 1074
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1074
    Illegal SpeI site found at 702
    Illegal PstI site found at 716
  • 1000
    COMPATIBLE WITH RFC[1000]