Difference between revisions of "Part:BBa K3505033"

 
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<partinfo>BBa_K3505033 short</partinfo>
 
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===Usage and Biology===
 
===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
 
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<partinfo>BBa_K3505033 SequenceAndFeatures</partinfo>
 
  
  
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===Design Notes===
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===Functional Parameters===
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The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in alpha1R vector <bbpart>BBa_K3505008</bbpart> and has overhangs compatible for Golden Braid cloning.
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<partinfo>BBa_K3505033 parameters</partinfo>
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[[Image:T--Thessaly--ANDERSON-TETR-PHOT.png|900px|thumb|none|<I><b>Figure 2.</b> The level A module of Tea-Off system : AndersonJ23115:RBS-TetR-Double terminator </i>]]
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===Verification of Cloning===
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[[File:T--Thessaly--Anderson--TETR-LEVEL1.png|700px|thumb|none|<i><b>Fig.1:</b>(U=Uncut C=Cut) Restriction digestion of T: AndersonJ23115-TetR-double terminator(C3-C4 ) with: HindIII (C3-C4) , Expected bands : 2847 + 836 bp ,Positive result : C3,C4</i>]]
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===Sequence and Features===
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<partinfo>BBa_K3505033 SequenceAndFeatures</partinfo>

Revision as of 14:03, 26 October 2020


pAndersonJ23115:RBS:tetR-terminator



Usage and Biology

Design Notes

The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in alpha1R vector BBa_K3505008 and has overhangs compatible for Golden Braid cloning.

Figure 2. The level A module of Tea-Off system : AndersonJ23115:RBS-TetR-Double terminator


Verification of Cloning

Fig.1:(U=Uncut C=Cut) Restriction digestion of T: AndersonJ23115-TetR-double terminator(C3-C4 ) with: HindIII (C3-C4) , Expected bands : 2847 + 836 bp ,Positive result : C3,C4


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 76
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 76
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 76
  • 1000
    COMPATIBLE WITH RFC[1000]