Difference between revisions of "Part:BBa K3527003"
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In E. coli, the expression level of PhoA gene is affected by the concentration of phosphate. BBa_K3527003 uses enhancer green fluorescence protein (EGFP) gene as the reporter gene to construct the fusion detection vector PhoA-EGFP, and then the sythetic generator can be used as the biosensor displays different fluorescence intensities according to the level of phosphorus content in the water, which can easily and quickly detect the content of phosphorus in different water bodies | In E. coli, the expression level of PhoA gene is affected by the concentration of phosphate. BBa_K3527003 uses enhancer green fluorescence protein (EGFP) gene as the reporter gene to construct the fusion detection vector PhoA-EGFP, and then the sythetic generator can be used as the biosensor displays different fluorescence intensities according to the level of phosphorus content in the water, which can easily and quickly detect the content of phosphorus in different water bodies | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | BBa K3527003 is a composite part constituting of enhanced green fluorescent protein gene under control of phoA promoter (basic parts BBa_K3527000). The similar design was seen for composite parts BBa_K1139201 and BBa_K737024. The part BBa_K737024 does not have experimental data showing its function and the part BBa_K1139201 has sufficient data showing its function but no practical application. | |
| − | + | [[File:T--Shanghai_HS-BBa_K3527000 fig 0.jpg|500px|thumb|center|Figure 1]] | |
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Revision as of 14:01, 26 October 2020
PhoA-signal peptide-EGFP
In E. coli, the expression level of PhoA gene is affected by the concentration of phosphate. BBa_K3527003 uses enhancer green fluorescence protein (EGFP) gene as the reporter gene to construct the fusion detection vector PhoA-EGFP, and then the sythetic generator can be used as the biosensor displays different fluorescence intensities according to the level of phosphorus content in the water, which can easily and quickly detect the content of phosphorus in different water bodies
Usage and Biology
BBa K3527003 is a composite part constituting of enhanced green fluorescent protein gene under control of phoA promoter (basic parts BBa_K3527000). The similar design was seen for composite parts BBa_K1139201 and BBa_K737024. The part BBa_K737024 does not have experimental data showing its function and the part BBa_K1139201 has sufficient data showing its function but no practical application.
