Difference between revisions of "Part:BBa K190021"
 
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Group: iGEM20_CSU_CHINA (2020-10-26)<br>
 
Group: iGEM20_CSU_CHINA (2020-10-26)<br>
 
Author: Hao Zhou<br>
 
Author: Hao Zhou<br>
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Summary: We used the SmtA protein constructed by Groningen's team in 2009 (https://parts.igem.org/Part:BBa_K190021)to adsorb cadmium ions from outside the cell. We have successfully expressed protein in E. coli DH5α and supplemented experimental data to demonstrate that SmtA can Improve cadmium tolerance of E. coli respectively.（Figure a、b）<br>
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Summary: We used the SmtA protein constructed by Groningen's team in 2009 to adsorb cadmium ions from outside the cell. We have successfully expressed protein in E. coli DH5α and supplemented experimental data to demonstrate that SmtA can Improve cadmium tolerance of E. coli respectively.（Figure a、b）<br>
 
<center>https://2020.igem.org/wiki/images/thumb/7/7b/T--CSU_CHINA--Contribution_SmtA.png/800px-T--CSU_CHINA--Contribution_SmtA.png</center><br>
 
<center>https://2020.igem.org/wiki/images/thumb/7/7b/T--CSU_CHINA--Contribution_SmtA.png/800px-T--CSU_CHINA--Contribution_SmtA.png</center><br>
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<center>Figure a：Western blotting results indicating the successful expression in E.Coli<br>
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Figure b：Growth curve after successful protein expression</center><br>
  
 
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Latest revision as of 13:18, 26 October 2020

SmtA

SmtA is a metallothionein that binds Zn (II) ions, it is able to bind 3-4 ions per protein.

Usage and Biology

The gene was functionally expressed in E. coli. The protein can be used to accumulate Zn(II) intracellular.

Supplementary Experiment

Group: iGEM20_CSU_CHINA (2020-10-26)
Author: Hao Zhou
Summary: We used the SmtA protein constructed by Groningen's team in 2009 to adsorb cadmium ions from outside the cell. We have successfully expressed protein in E. coli DH5α and supplemented experimental data to demonstrate that SmtA can Improve cadmium tolerance of E. coli respectively.（Figure a、b）

800px-T--CSU_CHINA--Contribution_SmtA.png

Figure a：Western blotting results indicating the successful expression in E.Coli
Figure b：Growth curve after successful protein expression

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 111
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 111
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 111
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 111
    Illegal AgeI site found at 139
  • 1000
    COMPATIBLE WITH RFC[1000]