Difference between revisions of "Part:BBa K190021"

Latest revision as of 13:18, 26 October 2020

SmtA

SmtA is a metallothionein that binds Zn (II) ions, it is able to bind 3-4 ions per protein.

Usage and Biology

The gene was functionally expressed in E. coli. The protein can be used to accumulate Zn(II) intracellular.

Supplementary Experiment

Group: iGEM20_CSU_CHINA (2020-10-26)
Author: Hao Zhou
Summary: We used the SmtA protein constructed by Groningen's team in 2009 to adsorb cadmium ions from outside the cell. We have successfully expressed protein in E. coli DH5α and supplemented experimental data to demonstrate that SmtA can Improve cadmium tolerance of E. coli respectively.（Figure a、b）

Figure a：Western blotting results indicating the successful expression in E.Coli
Figure b：Growth curve after successful protein expression

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 111
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 111
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 111
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 111
Illegal AgeI site found at 139
1000
COMPATIBLE WITH RFC[1000]

@@ Line 11: / Line 11: @@
 Group: iGEM20_CSU_CHINA (2020-10-26)<br>
 Author: Hao Zhou<br>
-Summary: <br>
+Summary: We used the SmtA protein constructed by Groningen's team in 2009 to adsorb cadmium ions from outside the cell. We have successfully expressed protein in E. coli DH5α and supplemented experimental data to demonstrate that SmtA can Improve cadmium tolerance of E. coli respectively.（Figure a、b）<br>
 <center>https://2020.igem.org/wiki/images/thumb/7/7b/T--CSU_CHINA--Contribution_SmtA.png/800px-T--CSU_CHINA--Contribution_SmtA.png</center><br>
+<center>Figure a：Western blotting results indicating the successful expression in E.Coli<br>
+Figure b：Growth curve after successful protein expression</center><br>
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