Difference between revisions of "Part:BBa K190021"
Howie Chow (Talk | contribs) |
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Group: iGEM20_CSU_CHINA (2020-10-26)<br> | Group: iGEM20_CSU_CHINA (2020-10-26)<br> | ||
Author: Hao Zhou<br> | Author: Hao Zhou<br> | ||
| − | Summary: | + | Summary: We used the SmtA protein constructed by Groningen's team in 2009 to adsorb cadmium ions from outside the cell. We have successfully expressed protein in E. coli DH5α and supplemented experimental data to demonstrate that SmtA can Improve cadmium tolerance of E. coli respectively.（Figure a、b）<br> |
| − | <center>https://2020.igem.org/wiki/images/7/7b/T--CSU_CHINA--Contribution_SmtA.png</center><br> | + | <center>https://2020.igem.org/wiki/images/thumb/7/7b/T--CSU_CHINA--Contribution_SmtA.png/800px-T--CSU_CHINA--Contribution_SmtA.png</center><br> |
| − | + | <center>Figure a：Western blotting results indicating the successful expression in E.Coli<br> | |
| + | Figure b：Growth curve after successful protein expression</center><br> | ||
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Latest revision as of 13:18, 26 October 2020
SmtA
SmtA is a metallothionein that binds Zn (II) ions, it is able to bind 3-4 ions per protein.
Usage and Biology
The gene was functionally expressed in E. coli. The protein can be used to accumulate Zn(II) intracellular.
Supplementary Experiment
Group: iGEM20_CSU_CHINA (2020-10-26)
Author: Hao Zhou
Summary: We used the SmtA protein constructed by Groningen's team in 2009 to adsorb cadmium ions from outside the cell. We have successfully expressed protein in E. coli DH5α and supplemented experimental data to demonstrate that SmtA can Improve cadmium tolerance of E. coli respectively.（Figure a、b）
Figure b：Growth curve after successful protein expression
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 111
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 111
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 111
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 111
Illegal AgeI site found at 139 - 1000COMPATIBLE WITH RFC[1000]