Difference between revisions of "Part:BBa K190021"
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Group: iGEM20_CSU_CHINA (2020-10-26)<br> | Group: iGEM20_CSU_CHINA (2020-10-26)<br> | ||
Author: Hao Zhou<br> | Author: Hao Zhou<br> | ||
− | Summary: | + | Summary: <br> |
− | <center>https://2020.igem.org/wiki/images/7/7b/T--CSU_CHINA--Contribution_SmtA.png</center><br> | + | <center>https://2020.igem.org/wiki/images/thumb/7/7b/T--CSU_CHINA--Contribution_SmtA.png/320px-T--CSU_CHINA--Contribution_SmtA.png</center><br> |
Revision as of 13:06, 26 October 2020
SmtA
SmtA is a metallothionein that binds Zn (II) ions, it is able to bind 3-4 ions per protein.
Usage and Biology
The gene was functionally expressed in E. coli. The protein can be used to accumulate Zn(II) intracellular.
Supplementary Experiment
Group: iGEM20_CSU_CHINA (2020-10-26)
Author: Hao Zhou
Summary:
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 111
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 111
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 111
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 111
Illegal AgeI site found at 139 - 1000COMPATIBLE WITH RFC[1000]