Difference between revisions of "Part:BBa K190021"

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Group: iGEM20_CSU_CHINA (2020-10-26)<br>
 
Group: iGEM20_CSU_CHINA (2020-10-26)<br>
 
Author: Hao Zhou<br>
 
Author: Hao Zhou<br>
Summary:  
+
Summary: <br>
<center>https://2020.igem.org/wiki/images/7/7b/T--CSU_CHINA--Contribution_SmtA.png</center><br>
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<center>https://2020.igem.org/wiki/images/thumb/7/7b/T--CSU_CHINA--Contribution_SmtA.png/320px-T--CSU_CHINA--Contribution_SmtA.png</center><br>
  
  

Revision as of 13:06, 26 October 2020

SmtA

SmtA is a metallothionein that binds Zn (II) ions, it is able to bind 3-4 ions per protein.

Usage and Biology

The gene was functionally expressed in E. coli. The protein can be used to accumulate Zn(II) intracellular.

Supplementary Experiment

Group: iGEM20_CSU_CHINA (2020-10-26)
Author: Hao Zhou
Summary:

320px-T--CSU_CHINA--Contribution_SmtA.png


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 111
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 111
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 111
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 111
    Illegal AgeI site found at 139
  • 1000
    COMPATIBLE WITH RFC[1000]