Difference between revisions of "Part:BBa K3606038"

 
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<partinfo>BBa_K3606038 short</partinfo>
 
<partinfo>BBa_K3606038 short</partinfo>
  
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OmpA_MBP for the test and verification of secretion led by NSP4.
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OmpA_MBP is constructed for the test and verification of secretion led by OmpA.
  
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<!-- Add more about the biology of this part here
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<!-- -->
 
===Usage and Biology===
 
===Usage and Biology===
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<p>MBP(Maltose Binding Protein) is a protein that normally expressed in the cytoplasm of E.coli. </p>
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<p>To test the efficiency of our 5 signal peptides for the secretion of the target protein, we fused our 5 signal peptides to MBP to compare their secretion capabilities. The signal peptides and corresponding serial numbers are listed below, through which the biology and characterization of each peptide are available.</p>
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<table>
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<tr>
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<th> Signal Peptide </th> <th> Number for Registry </th>
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</tr>
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<tr>
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<td> NSP4 </td> <td> BBa_K3606042 </td>
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</tr>
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<tr>
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<td> OmpA </td> <td> BBa_K3606043 </td>
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</tr>
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<tr>
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<td> DsbA </td> <td> BBa_K3606030 </td>
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</tr>
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<tr>
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<td> PelB </td> <td> BBa_K208004 </td>
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</tr>
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<tr>
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<td> PhoA </td> <td> BBa_K808028 </td>
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</tr>
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</table>
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<p>Moreover, we put the expression of secretion peptide guided MBP under the regulation of Ptac, an iPTG inducible promoter, to achieve better qualification of our secretion peptides.</p>
 +
 +
<p>To test the secretion efficiency of our 5 secretion peptides and compare their secretion capabilities, we also fused them to MBP(Maltose Binding Protein) that normally expressed in the cytoplasm of E.coli. cells. Driven by an iPTG inducible promoter Ptac, we can achieve quantified characterization of these secretion peptides.</p>
  
 
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<span class='h3bb'>Sequence and Features</span>
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<span class=h4>Sequence and Features</h4>
 
<partinfo>BBa_K3606038 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3606038 SequenceAndFeatures</partinfo>
  

Revision as of 12:42, 26 October 2020


PlacIq_lacI_Ptac driven OmpA-MBP

OmpA_MBP is constructed for the test and verification of secretion led by OmpA.

Usage and Biology

MBP(Maltose Binding Protein) is a protein that normally expressed in the cytoplasm of E.coli.

To test the efficiency of our 5 signal peptides for the secretion of the target protein, we fused our 5 signal peptides to MBP to compare their secretion capabilities. The signal peptides and corresponding serial numbers are listed below, through which the biology and characterization of each peptide are available.

Signal Peptide Number for Registry
NSP4 BBa_K3606042
OmpA BBa_K3606043
DsbA BBa_K3606030
PelB BBa_K208004
PhoA BBa_K808028

Moreover, we put the expression of secretion peptide guided MBP under the regulation of Ptac, an iPTG inducible promoter, to achieve better qualification of our secretion peptides.

To test the secretion efficiency of our 5 secretion peptides and compare their secretion capabilities, we also fused them to MBP(Maltose Binding Protein) that normally expressed in the cytoplasm of E.coli. cells. Driven by an iPTG inducible promoter Ptac, we can achieve quantified characterization of these secretion peptides.

Sequence and Features</h4>


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Unknown
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1233
    Illegal BglII site found at 1732
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1430