Difference between revisions of "Part:BBa K3505018"

Revision as of 10:36, 26 October 2020

sfGFP GB compatible with B3-B5

Level 0 sfGFP

Fig.1:sfGFP

Fig.2:sfGFP uder control of pTRC

Fig.3:The overhangs of this part in the Golden Braid Grammar

Usage and Biology

A reporter gene fluorescent protein

Excitation at 485 nm
Emission at 510 nm

Design Notes

The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in pUPD2 BBa_K3505007

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 149
1000
COMPATIBLE WITH RFC[1000]

@@ Line 11: / Line 11: @@
 [[File:T--Thessaly--sfpiato.jpeg|700px|thumb|none|<i><b>Fig.2:</b>sfGFP uder control of pTRC</i>]]
+[[File:T--Thessaly--GB-AATG-GCTT.jpeg|700px|thumb|none|<i><b>Fig.3:</b>The overhangs of this part in the Golden Braid Grammar</i>]]
 ===Usage and Biology===
@@ Line 19: / Line 21: @@
 ===Design Notes===
-<p>The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in pUPD2 <bbpart>BBa_K3505007<bbpart> and has overhangs compatible for Golden Braid cloning.
+The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in pUPD2 <bbpart>BBa_K3505007<bbpart>and has overhangs compatible for Golden Braid cloning. The CDS has position B3-B5.
-The CDS has position B3-B5. </p>
-<br>
-[[File:T--Thessaly--GB-AATG-GCTT.jpeg|700px|thumb|none|<i><b>Fig.3:</b>The overhangs of this part in the Golden Braid Grammar</i>]]