Difference between revisions of "Part:BBa K3505005"

Revision as of 10:29, 26 October 2020

TetR- Tet repressor GB compatible with B3-B5

TetR- tetR

Figure 1.The overhangs of this part in the Golden Braid Grammar.

Figure 2. The level 0 module : pupd2- TetR (illustration from SnapGene)

Fig.3:(U=Uncut C=Cut) Restriction digestion of TetR (C2 -C4) with : SpeI + EcoRI (C2 - C4) , Expected bands : 2047 + 680 bp Positive result : C4

Assembly Compatibility:

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K3505005 short</partinfo>
 TetR- tetR
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
@@ Line 16: / Line 17: @@
 ===Verification of Cloning===
-[[File:T--Thessaly--tetr-level0-digestion.png|700px|thumb|none|<i><b>Fig.1:</b>(U=Uncut C=Cut) Restriction digestion of TetR (C2 -C4) with :
+[[File:T--Thessaly--tetr-level0-digestion.png|700px|thumb|none|<i><b>Fig.3:</b>(U=Uncut C=Cut) Restriction digestion of TetR (C2 -C4) with :
 SpeI + EcoRI (C2 - C4) , Expected bands : 2047 + 680 bp
 Positive result : C4 </i>]]
-<!-- -->
-<span class='h3bb'>Sequence and Features</span>
-<partinfo>BBa_K3505005 SequenceAndFeatures</partinfo>
+===Sequence and Features===
-<!-- Uncomment this to enable Functional Parameter display
+<partinfo>BBa_K3505005 SequenceAndFeatures</partinfo>
-===Functional Parameters===
-<partinfo>BBa_K3505005 parameters</partinfo>
-<!-- -->