Difference between revisions of "Part:BBa K3505005"
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===Usage and Biology=== | ===Usage and Biology=== | ||
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+ | <p style="text-align: center;"> | ||
+ | [[Image: T--Thessaly--GB-AATG-GCTT.jpeg|900px|thumb|none|<i><b>Figure 1.</b>The overhangs of this part in the Golden Braid Grammar.</i>]] | ||
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+ | [[Image:T--Thessaly--tetR-snap.png |900px|thumb|none|<I><b>Figure 2.</b> The level 0 module : pupd2- TetR (illustration from SnapGene)</i>]] | ||
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+ | ===Verification of Cloning=== | ||
+ | [[File:T--Thessaly--tetr-level0-digestion.png|700px|thumb|none|<i><b>Fig.1:</b>(U=Uncut C=Cut) Restriction digestion of TetR (C2 -C4) with : | ||
+ | SpeI + EcoRI (C2 - C4) , Expected bands : 2047 + 680 bp | ||
+ | Positive result : C4 �</i>]] | ||
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Revision as of 10:27, 26 October 2020
TetR- Tet repressor GB compatible with B3-B5
TetR- tetR
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 4
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 4
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 4
- 1000COMPATIBLE WITH RFC[1000]