Difference between revisions of "Part:BBa K3562014"

 
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===Usage and Biology===
 
  
 
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<partinfo>BBa_K3562014 parameters</partinfo>
 
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===Usage and Biology===
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<p>
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Chemokines are a family of small chemotactic cytokines, and the name is derived from their ability to induce directed chemotaxis in nearby responsive cells. All chemokines possess a number of conserved cysteine residues involved in intramolecular disulfide bond formation. Some chemokines can be induced during an immune response to recruit cells of the immune system to a site of infection, while others are considered homeostatic and are involved in controlling the migration of cells during normal processes of tissue maintenance or development. Chemokines are found in all vertebrates, some viruses and some bacteria.
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</p>
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<p>
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MIP1-alpha/CCL3 is a monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).
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</p>
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<p>
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In our project, chemokines are used to attract immune cells to accumulate to the lesion faster, replacing the original role of quorum sensing factors in the immune response.
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</p>
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===Results===
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<p>
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We used the Transwell experiment combined with flow cytometry to verify the chemotactic ability of this chemokine on the THP1 cell line.(Fig.1)
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</p>
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[[Image:CCL3 fig1.png|frame|'''Figure 1''' Chemotaxis of CCL3 to THP1(shown by cell number). Chemokine concentration is 10 nmol/l. (n=3)]]
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<p>
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Besides, we also tested the activity of six other chemokines.(Fig.2)
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</p>
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[[Image:chemokines.png|frame|'''Figure 2''' Chemotaxis index(CI) of 7 chemokines to THP1.(n=3)]]
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===Reference===
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[1][https://www.ebi.ac.uk/QuickGO/term/GO:0008009 Chemokine activity definition]
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<br/>
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[2][https://www.uniprot.org/uniprot/P10147 UniProtKB - P10147 (CCL3_HUMAN)]

Revision as of 09:57, 26 October 2020


CCL3

Monokine with inflammatory and chemokinetic properties.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

Chemokines are a family of small chemotactic cytokines, and the name is derived from their ability to induce directed chemotaxis in nearby responsive cells. All chemokines possess a number of conserved cysteine residues involved in intramolecular disulfide bond formation. Some chemokines can be induced during an immune response to recruit cells of the immune system to a site of infection, while others are considered homeostatic and are involved in controlling the migration of cells during normal processes of tissue maintenance or development. Chemokines are found in all vertebrates, some viruses and some bacteria.

MIP1-alpha/CCL3 is a monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).

In our project, chemokines are used to attract immune cells to accumulate to the lesion faster, replacing the original role of quorum sensing factors in the immune response.

Results

We used the Transwell experiment combined with flow cytometry to verify the chemotactic ability of this chemokine on the THP1 cell line.(Fig.1)

Figure 1 Chemotaxis of CCL3 to THP1(shown by cell number). Chemokine concentration is 10 nmol/l. (n=3)

Besides, we also tested the activity of six other chemokines.(Fig.2)

Figure 2 Chemotaxis index(CI) of 7 chemokines to THP1.(n=3)

Reference

[1]Chemokine activity definition
[2]UniProtKB - P10147 (CCL3_HUMAN)