Difference between revisions of "Part:BBa K3429003"
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<partinfo>BBa_K3429003 short</partinfo> | <partinfo>BBa_K3429003 short</partinfo> | ||
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| − | We use this <b>flexible glycine-serine linker</b> (GGGGSGGGGS) to create TasA fusion proteins with CotA | + | We use this <b>flexible glycine-serine linker</b> (GGGGSGGGGS) to create TasA fusion proteins with CotA, CuO and EreB. Huang et al. fused TasA to superfolder GFP by using this protein linker without impairing TasA or sfGFP function<sup>[1]</sup>. |
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<h2>References</h2> | <h2>References</h2> | ||
Latest revision as of 09:30, 26 October 2020
Protein Linker for fusion proteins: ARGGGGSGGGGSGS
We use this flexible glycine-serine linker (GGGGSGGGGS) to create TasA fusion proteins with CotA, CuO and EreB. Huang et al. fused TasA to superfolder GFP by using this protein linker without impairing TasA or sfGFP function[1].
References
[1] Huang, J., Liu, S., Zhang, C. et al., Programmable and printable Bacillus subtilis biofilms as engineered living materials. Nat Chem Biol 15, 34–41, (2019), https://doi.org/10.1038/s41589-018-0169-2
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]