Difference between revisions of "Part:BBa K3429003"

 
 
(7 intermediate revisions by one other user not shown)
Line 3: Line 3:
 
<partinfo>BBa_K3429003 short</partinfo>
 
<partinfo>BBa_K3429003 short</partinfo>
  
−
linker for fusion proteins of TasA and sfGFP or enzymes
+
<br>
 +
 
 +
We use this <b>flexible glycine-serine linker</b> (GGGGSGGGGS) to create TasA fusion proteins with CotA, CuO and EreB. Huang et al. fused TasA to superfolder GFP by using this protein linker without impairing TasA or sfGFP function<sup>[1]</sup>.
 +
 
 +
 
 +
<h2>References</h2>
 +
 
 +
[1] Huang, J., Liu, S., Zhang, C. et al., Programmable and printable Bacillus subtilis biofilms as engineered living materials. Nat Chem Biol 15, 34–41, (2019), https://doi.org/10.1038/s41589-018-0169-2
 +
 
 +
<br>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 09:30, 26 October 2020


Protein Linker for fusion proteins: ARGGGGSGGGGSGS


We use this flexible glycine-serine linker (GGGGSGGGGS) to create TasA fusion proteins with CotA, CuO and EreB. Huang et al. fused TasA to superfolder GFP by using this protein linker without impairing TasA or sfGFP function[1].


References

[1] Huang, J., Liu, S., Zhang, C. et al., Programmable and printable Bacillus subtilis biofilms as engineered living materials. Nat Chem Biol 15, 34–41, (2019), https://doi.org/10.1038/s41589-018-0169-2


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]