Difference between revisions of "Part:BBa K3429000"
 
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IPTG-inducible promoter as used in Huang et al. for TasA fusion protein expression. <sup> [1] </sup> We designed plasmid X [link to plasmid] encoding gene X regulated by the grac promoter.
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IPTG-inducible promoter as used in Huang et al. for TasA fusion protein expression<sup>[1]</sup>. We decided to use the same promoter and terminator system as Huang et al. did for their TasA-sfGFP as well as our TasA-CotA/CuO/EreB fusion proteins.
  
  

Latest revision as of 09:15, 26 October 2020


Pgrac promoter


IPTG-inducible promoter as used in Huang et al. for TasA fusion protein expression[1]. We decided to use the same promoter and terminator system as Huang et al. did for their TasA-sfGFP as well as our TasA-CotA/CuO/EreB fusion proteins.


References

[1] Huang, J., Liu, S., Zhang, C. et al., Programmable and printable Bacillus subtilis biofilms as engineered living materials. Nat Chem Biol 15, 34–41, (2019), https://doi.org/10.1038/s41589-018-0169-2



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]