Difference between revisions of "Part:BBa K3593011:Design"

 
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===Design Notes===
 
===Design Notes===
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add antigen tag that is more specific than 6*His tag
+
you may add antigen tag that is more specific than 6*His tag.
−
 
+
you may mutate this gene to reach a higher specificity.
−
 
+
  
 
===Source===
 
===Source===
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===References===
 
===References===
 +
 +
1. Zhang X, He K, Zhao R, Feng T, Wei D. Development of a Single Chain Variable Fragment Antibody and Application as Amatoxin Recognition Molecule in Surface Plasmon Resonance Sensors. Food Anal Methods. 2016. doi:10.1007/s12161-016-0509-3
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2. SAbPred: ABodyBuilder http://opig.stats.ox.ac.uk/webapps/newsabdab/sabpred/abodybuilder/

Latest revision as of 04:47, 26 October 2020


Single-Chain Fragment Variable (anti α-amatinin)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 98
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

you may add antigen tag that is more specific than 6*His tag. you may mutate this gene to reach a higher specificity.

Source

Artificially synthesized

References

1. Zhang X, He K, Zhao R, Feng T, Wei D. Development of a Single Chain Variable Fragment Antibody and Application as Amatoxin Recognition Molecule in Surface Plasmon Resonance Sensors. Food Anal Methods. 2016. doi:10.1007/s12161-016-0509-3

2. SAbPred: ABodyBuilder http://opig.stats.ox.ac.uk/webapps/newsabdab/sabpred/abodybuilder/