Difference between revisions of "Part:BBa K3552010"

 
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__NOTOC__
 
 
<partinfo>BBa_K3552010 short</partinfo>
 
<partinfo>BBa_K3552010 short</partinfo>
  
 
The complete circuit including a GsPilA and its generator with EHEC genes which is the gene that we mutated. This part is in the part collection where we have 12 genes that code for the base generator of pilA.  
 
The complete circuit including a GsPilA and its generator with EHEC genes which is the gene that we mutated. This part is in the part collection where we have 12 genes that code for the base generator of pilA.  
  
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The part collection includes: Parts that are different kinds of type 4 pilus: BBa_K3552000 BBa_K3552001 BBa_K3552002. Parts that are the generator of the type 4 pilus: BBa_K3552003 BBa_K3552004 BBa_K3552005 BBa_K3552006 BBa_K3552007 BBa_K3552008 BBa_K3552018 BBa_K3552019 BBa_K3552020 BBa_K3552021 BBa_K3552022 BBa_K3552023 BBa_K3552024 BBa_K3552025 BBa_K3552026 BBa_K3552027 BBa_K3552028 BBa_K3552029. Parts that are a complete circuit: BBa_K3552009 BBa_K3552010 BBa_K3552011 BBa_K3552012.
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The part collection includes:
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Parts that are different kinds of type 4 pilus:
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<partinfo>BBa_K3552003</partinfo> 
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<partinfo>BBa_K3552004</partinfo>
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<partinfo>BBa_K3552005</partinfo>
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<partinfo>BBa_K3552006</partinfo>
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<partinfo>BBa_K3552007</partinfo>
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<partinfo>BBa_K3552008</partinfo>
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<partinfo>BBa_K3552018</partinfo> 
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<partinfo>BBa_K3552019</partinfo> 
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<partinfo>BBa_K3552020</partinfo> 
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<partinfo>BBa_K3552021</partinfo> 
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<partinfo>BBa_K3552022</partinfo> 
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<partinfo>BBa_K3552023</partinfo> 
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<partinfo>BBa_K3552024</partinfo> 
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<partinfo>BBa_K3552025</partinfo> 
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<partinfo>BBa_K3552026</partinfo> 
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<partinfo>BBa_K3552027</partinfo> 
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<partinfo>BBa_K3552028</partinfo> 
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<partinfo>BBa_K3552029</partinfo> 
  
 
Our part collection can instruct other teams to designed new rechargeable pilus and substitution of different major pilin.
 
Our part collection can instruct other teams to designed new rechargeable pilus and substitution of different major pilin.
  
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Characterization:
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<span class='h3bb'>'''Sequence and Features'''</span>
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<partinfo>BBa_K3552000 SequenceAndFeatures</partinfo>
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==Characterization==
 
We expressed this complete circuit plasmid in BL21 and verified the function through Western Blot. We also uses the extracted pili to make batteries and measure the voltage and current.
 
We expressed this complete circuit plasmid in BL21 and verified the function through Western Blot. We also uses the extracted pili to make batteries and measure the voltage and current.
  
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<!-- Add more about the biology of this part here
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<partinfo>BBa_K3552000 parameters</partinfo>
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3552010 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K3552010 parameters</partinfo>
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Revision as of 03:52, 26 October 2020


GsPilA expressed with EHEC similar generatore

The complete circuit including a GsPilA and its generator with EHEC genes which is the gene that we mutated. This part is in the part collection where we have 12 genes that code for the base generator of pilA.

The part collection includes: Parts that are different kinds of type 4 pilus: BBa_K3552003 BBa_K3552004 BBa_K3552005 BBa_K3552006 BBa_K3552007 BBa_K3552008 BBa_K3552018 BBa_K3552019 BBa_K3552020 BBa_K3552021 BBa_K3552022 BBa_K3552023 BBa_K3552024 BBa_K3552025 BBa_K3552026 BBa_K3552027 BBa_K3552028 BBa_K3552029

Our part collection can instruct other teams to designed new rechargeable pilus and substitution of different major pilin.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization

We expressed this complete circuit plasmid in BL21 and verified the function through Western Blot. We also uses the extracted pili to make batteries and measure the voltage and current.