Difference between revisions of "Part:BBa K3552000"

 
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__NOTOC__
 
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<partinfo>BBa_K3552000 short</partinfo>
 
<partinfo>BBa_K3552000 short</partinfo>
  
 
GsPilA is a type 4 pilus generally on Geobacter sulfurreducens which can conduct electricity. This part is in the part collection where we provide different conductive pilus based on the same pilin generator. By these different type 4 pilus we can compare the qualities.
 
GsPilA is a type 4 pilus generally on Geobacter sulfurreducens which can conduct electricity. This part is in the part collection where we provide different conductive pilus based on the same pilin generator. By these different type 4 pilus we can compare the qualities.
  
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GsPilA are fimbrial protein which normally grow on Geobacter sulfurreducens. They are type 4 pili which are long and thin which display on the cell surface membrane. They can promote adherence, motility and transport functions in the bacteria. They are mainly built as helical polymers of a single subunit called the major pilin. These pilins are initially in the plasma membrane and the N-terminal is positively charged. The mature pilins will be extracted through the membrane by the pilin generator.
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The part collection includes:
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Parts that are different kinds of type 4 pilus:
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<partinfo>BBa_K3552003</partinfo> 
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<partinfo>BBa_K3552004</partinfo>
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<partinfo>BBa_K3552005</partinfo>
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<partinfo>BBa_K3552006</partinfo>
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<partinfo>BBa_K3552007</partinfo>
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<partinfo>BBa_K3552008</partinfo>
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<partinfo>BBa_K3552018</partinfo> 
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<partinfo>BBa_K3552019</partinfo> 
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<partinfo>BBa_K3552020</partinfo> 
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<partinfo>BBa_K3552021</partinfo> 
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<partinfo>BBa_K3552022</partinfo> 
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<partinfo>BBa_K3552023</partinfo> 
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<partinfo>BBa_K3552024</partinfo> 
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<partinfo>BBa_K3552025</partinfo> 
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<partinfo>BBa_K3552026</partinfo> 
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<partinfo>BBa_K3552027</partinfo> 
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<partinfo>BBa_K3552028</partinfo> 
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<partinfo>BBa_K3552029</partinfo> 
  
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<!-- Add more about the biology of this part here
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Our part collection can instruct other teams to designed new rechargeable pilus and substitution of different major pilin.
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===Usage and Biology===
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<span class='h3bb'>'''Sequence and Features'''</span>
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<span class='h3bb'>Sequence and Features</span>
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<partinfo> BBa_K3552000 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K3552000 SequenceAndFeatures</partinfo>
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==Usage and Biology==
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GsPilA are fimbrial protein which normally grow on Geobacter sulfurreducens. They are type 4 pili which are long and thin which display on the cell surface membrane. They can promote adherence, motility and transport functions in the bacteria. They are mainly built as helical polymers of a single subunit called the major pilin. These pilins are initially in the plasma membrane and the N-terminal is positively charged. The mature pilins will be extracted through the membrane by the pilin generator.
  
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==Characterization==
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We first constructed the GsPilA by SOE PCR through 8 different primers to maintain the exactly same sequence as in the Geobacter sulfurreducens. After the final construction of the generator of PilA, we added six His-tag on the GsPilA and expressed the gene in vivo. After 48 hours of growth, we extracted the pili and verified the existence of GsPilA on the outer cell through Western Blot. We also do electronic speculum and see the clear image of the pili in the solution.
  
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<!-- Uncomment this to enable Functional Parameter display
 
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===Functional Parameters===
 
 
<partinfo>BBa_K3552000 parameters</partinfo>
 
<partinfo>BBa_K3552000 parameters</partinfo>
 
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Revision as of 00:52, 26 October 2020


GsPilA

GsPilA is a type 4 pilus generally on Geobacter sulfurreducens which can conduct electricity. This part is in the part collection where we provide different conductive pilus based on the same pilin generator. By these different type 4 pilus we can compare the qualities.

The part collection includes: Parts that are different kinds of type 4 pilus: BBa_K3552003 BBa_K3552004 BBa_K3552005 BBa_K3552006 BBa_K3552007 BBa_K3552008 BBa_K3552018 BBa_K3552019 BBa_K3552020 BBa_K3552021 BBa_K3552022 BBa_K3552023 BBa_K3552024 BBa_K3552025 BBa_K3552026 BBa_K3552027 BBa_K3552028 BBa_K3552029

Our part collection can instruct other teams to designed new rechargeable pilus and substitution of different major pilin.

Sequence and Features No part name specified with partinfo tag.

Usage and Biology

GsPilA are fimbrial protein which normally grow on Geobacter sulfurreducens. They are type 4 pili which are long and thin which display on the cell surface membrane. They can promote adherence, motility and transport functions in the bacteria. They are mainly built as helical polymers of a single subunit called the major pilin. These pilins are initially in the plasma membrane and the N-terminal is positively charged. The mature pilins will be extracted through the membrane by the pilin generator.

Characterization

We first constructed the GsPilA by SOE PCR through 8 different primers to maintain the exactly same sequence as in the Geobacter sulfurreducens. After the final construction of the generator of PilA, we added six His-tag on the GsPilA and expressed the gene in vivo. After 48 hours of growth, we extracted the pili and verified the existence of GsPilA on the outer cell through Western Blot. We also do electronic speculum and see the clear image of the pili in the solution.