Difference between revisions of "Part:BBa K077126"

 
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This part was used to prove that induction with HSL is working.
 
This part was used to prove that induction with HSL is working.
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Input: 3OC6HSL
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Output: LacZalpha
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 19:43, 29 October 2008

Test construct for HSL/LuxR promotor, with LacZalpha output

This is the test construct for the HSL/LuxR promotor BBa_R0062, with a LacZalpha output. This part is build up from BBa_R0062 and BBa_K077124. This part is very similar to BBa_T9003 the only difference is the order of the sub parts.

Comparinghsl.jpg

The image above shows the LacZ output of different parts from the registry. Important is that T9003 is placed in a low copy plasmid (pSB3K3) and the other parts are placed in a high copy plasmid (pSB1A2).

This part was used to prove that induction with HSL is working.

Input: 3OC6HSL

Output: LacZalpha

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Unknown
  • 1000
    COMPATIBLE WITH RFC[1000]