Difference between revisions of "Part:BBa K3562002"
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[[Image:pLasRV fig1.png|frame|'''Figure 1''' Characterization of inducible promoter pLasRV in cell-free systems and E. coli [1]]]
 
[[Image:pLasRV fig1.png|frame|'''Figure 1''' Characterization of inducible promoter pLasRV in cell-free systems and E. coli [1]]]
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[[Image:pLasRV fig2.png|frame|'''Figure 2''' Overview of the LasRV cell-free biosensor [2]]]
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[[Image:pLasRV fig3.png|frame|'''Figure 3''' Detection of sputum sample using the LasRV cell-free biosensor [2]]]
  
 
===Reference===
 
===Reference===
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[1]
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[1]Chappel J et al., Validation of an entirely in vitro approach for rapid prototyping of DNA regulatory elements for synthetic biology. Nucleic Acids Research, 41 (2013): 3471–3481.
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[2]Wen KY et al., A Cell-Free Biosensor for Detecting Quorum Sensing Molecules in P. aeruginosa-Infected Respiratory Samples. ACS Synth. Biol., 6 (2017): 2293-2301.

Revision as of 20:59, 25 October 2020


pLasRV

las promoter from Pseudomonas aeruginosa

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 14


Usage and Biology

We found this well-characterized promoter pLasRV from P. aeruginosa cloned by Paul Freemont group in Imperial College, with in vivo and in vitro expression data thoroughly presented in the research articles [1]. Notably, researchers developed a cell-free biosensor based on this promoter to detect the concentration of OdDHL in sputum samples [2]. More attempts to work on this inducible promoter in iGEM will deepen the understanding of quorum sensing in P. aeruginosa and facilitate the exploitation of biomarker-based biosensors.

Figure 1 Characterization of inducible promoter pLasRV in cell-free systems and E. coli [1]
Figure 2 Overview of the LasRV cell-free biosensor [2]
Figure 3 Detection of sputum sample using the LasRV cell-free biosensor [2]

Reference

[1]Chappel J et al., Validation of an entirely in vitro approach for rapid prototyping of DNA regulatory elements for synthetic biology. Nucleic Acids Research, 41 (2013): 3471–3481. [2]Wen KY et al., A Cell-Free Biosensor for Detecting Quorum Sensing Molecules in P. aeruginosa-Infected Respiratory Samples. ACS Synth. Biol., 6 (2017): 2293-2301.