Difference between revisions of "Part:BBa K3562002"

Revision as of 18:52, 25 October 2020

pLasRV

las promoter from Pseudomonas aeruginosa

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 14

Usage and Biology

We found this well-characterized promoter pLasRV from P. aeruginosa cloned by Paul Freemont group in Imperial College, with in vivo and in vitro expression data thoroughly presented in the research articles [1]. Notably, researchers developed a cell-free biosensor based on this promoter to detect the concentration of OdDHL in sputum samples [2]. More attempts to work on this inducible promoter in iGEM will deepen the understanding of quorum sensing in P. aeruginosa and facilitate the exploitation of biomarker-based biosensors.

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3562002 short</partinfo>
-las promoter from Pseudomonas aeruginosa
+las promoter from ''Pseudomonas aeruginosa''
 <!-- Add more about the biology of this part here
-===Usage and Biology===
 <!-- -->
@@ Line 17: / Line 17: @@
 <partinfo>BBa_K3562002 parameters</partinfo>
 <!-- -->
+===Usage and Biology===
+<p>
+We found this well-characterized promoter pLasRV from ''P. aeruginosa'' cloned by Paul Freemont group in Imperial College, with in vivo and in vitro expression data thoroughly presented in the research articles [1]. Notably, researchers developed a cell-free biosensor based on this promoter to detect the concentration of OdDHL in sputum samples [2]. More attempts to work on this inducible promoter in iGEM will deepen the understanding of quorum sensing in P. aeruginosa and facilitate the exploitation of biomarker-based biosensors.
+</p>