Difference between revisions of "Part:BBa K3425131"

 
 
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<partinfo>BBa_K3425131 short</partinfo>
 
<partinfo>BBa_K3425131 short</partinfo>
  
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The yeaR promoter is a part of the yeaR-yoaG operon which plays a role in nitrate and nitrite-responsive control in E. coli. PyeaR is activated by the response regulator NarL and repressed by the protein NsrR. Since pyeaR is natively present in E. coli, it can be used as a part of the two-component system, NarX/NarL. An ribosomal binding site (RBS), terminator and GFP sequence was added to show visual color change upon activation of pYeaR.  
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This composite part has a codon optimized GFP (BBa_K3425132) under the control of the yeaR promoter (BBa_K3425130). In the presence of nitrates or nitrites, the ''E. coli'' NarX-NarL two-component system is activated, which in turn upregulates PyeaR. This triggers expression of the GFP, resulting in a visual color change.
  
 
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'>'''Sequence and Features'''</span>
 
<partinfo>BBa_K3425131 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3425131 SequenceAndFeatures</partinfo>
  

Latest revision as of 18:51, 25 October 2020


PyeaR GFP

This composite part has a codon optimized GFP (BBa_K3425132) under the control of the yeaR promoter (BBa_K3425130). In the presence of nitrates or nitrites, the E. coli NarX-NarL two-component system is activated, which in turn upregulates PyeaR. This triggers expression of the GFP, resulting in a visual color change.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 735
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]