Difference between revisions of "Part:BBa K3380101:Experience"

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(→‎Applications of BBa_K3380101)
 
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===Applications of BBa_K3380101===
 
===Applications of BBa_K3380101===
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The F30 Upstream scaffold (BBa_K3380101) was used in tandem with F30 Downstream scaffold (BBa_K3380102) flanking the iSpinach fluorescent RNA aptamer (BBa_K3380150) under the class III T7 RNA polymerase strong promoter (BBa_z0251). The construct [https://parts.igem.org/Part:BBa_K3380500 BBa_K3380500] was synthesized to test if the iSpinach RNA aptamer would exhibit fluorescence when adding DFHBI fluorophore. The construct was tested in  optimised transcription/detection buffer (OTDB). The OTDB buffer was prepared as a 10X stock as follows: Tris base 400 mM pH 7.5 adjusted with HCl (Sigma-Aldrich, #T1503); MgCl2 6(H2O) 60 mM (Sigma-Aldrich, #M2670); DTT 100 mM (Melford, #MB1015) and Spermidine 20 mM (Alfa Aesar, #A19096.03).
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The F30 Upstream scaffold (BBa_K3380101) was used in tandem with F30 Downstream scaffold (BBa_K3380102) flanking the iSpinach fluorescent RNA aptamer (BBa_K3380150) under the class III T7 RNA polymerase strong promoter (BBa_z0251). The constructs BBa_K3380500, BBa_K3380600 and BBa_K3380603 were synthesized to test if the iSpinach RNA aptamer would exhibit fluorescence when adding DFHBI fluorophore. The constructs were tested in  optimised transcription/detection buffer (OTDB). The OTDB buffer was prepared as a 10X stock as follows: Tris base 400 mM pH 7.5 adjusted with HCl (Sigma-Aldrich, #T1503); MgCl2 6(H2O) 60 mM (Sigma-Aldrich, #M2670); DTT 100 mM (Melford, #MB1015) and Spermidine 20 mM (Alfa Aesar, #A19096.03).
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 18:50, 25 October 2020


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Applications of BBa_K3380101

The F30 Upstream scaffold (BBa_K3380101) was used in tandem with F30 Downstream scaffold (BBa_K3380102) flanking the iSpinach fluorescent RNA aptamer (BBa_K3380150) under the class III T7 RNA polymerase strong promoter (BBa_z0251). The constructs BBa_K3380500, BBa_K3380600 and BBa_K3380603 were synthesized to test if the iSpinach RNA aptamer would exhibit fluorescence when adding DFHBI fluorophore. The constructs were tested in optimised transcription/detection buffer (OTDB). The OTDB buffer was prepared as a 10X stock as follows: Tris base 400 mM pH 7.5 adjusted with HCl (Sigma-Aldrich, #T1503); MgCl2 6(H2O) 60 mM (Sigma-Aldrich, #M2670); DTT 100 mM (Melford, #MB1015) and Spermidine 20 mM (Alfa Aesar, #A19096.03).

User Reviews

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