Difference between revisions of "Part:BBa K3589208"
Line 3: | Line 3: | ||
<partinfo>BBa_K3589208 short</partinfo> | <partinfo>BBa_K3589208 short</partinfo> | ||
− | + | <html> | |
+ | <p> | ||
+ | This composite part contains a spectinomycin resistance (<a href="https://parts.igem.org/Part:BBa_K3002102">BBa_K3002102</a>) and the wildtype laccase from an uncultured marine bacteria (<a href="https://parts.igem.org/Part:BBa_K3589109">BBa_K3589109</a>) fused with an 3xHA-tag (<a href="https://parts.igem.org/Part:BBa_K3002017">BBa_K3002017</a>) for easy detection via HA-antibody. | ||
+ | </p> | ||
+ | </html> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 17:38, 25 October 2020
Level 2 spectinomycin resistance + marLac_3xHA
This composite part contains a spectinomycin resistance (BBa_K3002102) and the wildtype laccase from an uncultured marine bacteria (BBa_K3589109) fused with an 3xHA-tag (BBa_K3002017) for easy detection via HA-antibody.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2401
Illegal PstI site found at 3728 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2401
Illegal NheI site found at 2665
Illegal PstI site found at 3728 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2401
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2401
Illegal PstI site found at 3728 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2401
Illegal PstI site found at 3728
Illegal NgoMIV site found at 1401
Illegal NgoMIV site found at 1584
Illegal NgoMIV site found at 1694
Illegal NgoMIV site found at 3588
Illegal NgoMIV site found at 4307
Illegal NgoMIV site found at 4758
Illegal NgoMIV site found at 5311 - 1000COMPATIBLE WITH RFC[1000]