Difference between revisions of "Part:BBa K3589204"

 
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This composite part contains the PAR-promoter (<a href="https://parts.igem.org/Part:BBa_K3002027">BBa_K3002027</a>) in combination with the RPL23-Terminator (<a href="https://parts.igem.org/Part:BBa_K3002006">BBa_K3002006</a>), cCA secretion signal (<a href="https://parts.igem.org/Part:BBa_K3002007">BBa_K3002007</a>), SP20-HA-RGS-8His-tag (<a href="https://parts.igem.org/Part:BBa_K3589150">BBa_K3589150</a>) and the coding sequence of the wildtype laccase from an uncultured marine bacteria (<a href="https://parts.igem.org/Part:BBa_K3589109">BBa_K3589109</a>)  plus the MoClo connectors for positions B1-B2 (<a href="https://parts.igem.org/Part:BBa_K3002302">BBa_K3002302</a>), B2-B3 (<a href="https://parts.igem.org/Part:BBa_K3002303">BBa_K3002303</a>), B4-B5 (<a href="https://parts.igem.org/Part:BBa_K3002304">BBa_K3002304</a>) and B5-B6 (<a href="https://parts.igem.org/Part:BBa_K3002305">BBa_K3002305</a>).
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<!-- Add more about the biology of this part here
 
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Revision as of 17:17, 25 October 2020


Level 1 Wildtype marLac + cCA + SP20-HA-RGS-8His

This composite part contains the PAR-promoter (BBa_K3002027) in combination with the RPL23-Terminator (BBa_K3002006), cCA secretion signal (BBa_K3002007), SP20-HA-RGS-8His-tag (BBa_K3589150) and the coding sequence of the wildtype laccase from an uncultured marine bacteria (BBa_K3589109) plus the MoClo connectors for positions B1-B2 (BBa_K3002302), B2-B3 (BBa_K3002303), B4-B5 (BBa_K3002304) and B5-B6 (BBa_K3002305).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1379
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 249
    Illegal PstI site found at 1379
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1379
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1379
    Illegal NgoMIV site found at 1239
    Illegal NgoMIV site found at 1958
    Illegal NgoMIV site found at 2409
    Illegal NgoMIV site found at 3055
  • 1000
    COMPATIBLE WITH RFC[1000]