Difference between revisions of "Part:BBa K3589203"

 
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This composite part contains the PAR-promoter (<a href="https://parts.igem.org/Part:BBa_K3002027">BBa_K3002027</a>) in combination with the RPL23-Terminator (<a href="https://parts.igem.org/Part:BBa_K3002006">BBa_K3002006</a>), cCA secretion signal (<a href="https://parts.igem.org/Part:BBa_K3002007">BBa_K3002007</a>), SP20-HA-RGS-8His-tag (<a href="https://parts.igem.org/Part:BBa_K3589150">BBa_K3589150</a>) and the coding sequence of the mutant laccase from <I>Botrytis aclada</i> (<a href="https://parts.igem.org/Part:BBa_K3589108">BBa_K3589108</a>)  plus the MoClo connectors for positions B1-B2 (<a href="https://parts.igem.org/Part:BBa_K3002302">BBa_K3002302</a>), B2-B3 (<a href="https://parts.igem.org/Part:BBa_K3002303">BBa_K3002303</a>), B4-B5 (<a href="https://parts.igem.org/Part:BBa_K3002304">BBa_K3002304</a>) and B5-B6 (<a href="https://parts.igem.org/Part:BBa_K3002305">BBa_K3002305</a>).
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 17:16, 25 October 2020


Level 1Mutant BaLac + cCA + SP20-HA-RGS-8His

This composite part contains the PAR-promoter (BBa_K3002027) in combination with the RPL23-Terminator (BBa_K3002006), cCA secretion signal (BBa_K3002007), SP20-HA-RGS-8His-tag (BBa_K3589150) and the coding sequence of the mutant laccase from Botrytis aclada (BBa_K3589108) plus the MoClo connectors for positions B1-B2 (BBa_K3002302), B2-B3 (BBa_K3002303), B4-B5 (BBa_K3002304) and B5-B6 (BBa_K3002305).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1170
    Illegal PstI site found at 1257
    Illegal PstI site found at 1321
    Illegal PstI site found at 1693
    Illegal PstI site found at 1981
    Illegal PstI site found at 2058
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 249
    Illegal PstI site found at 1170
    Illegal PstI site found at 1257
    Illegal PstI site found at 1321
    Illegal PstI site found at 1693
    Illegal PstI site found at 1981
    Illegal PstI site found at 2058
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1617
    Illegal BglII site found at 2576
    Illegal BamHI site found at 1956
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1170
    Illegal PstI site found at 1257
    Illegal PstI site found at 1321
    Illegal PstI site found at 1693
    Illegal PstI site found at 1981
    Illegal PstI site found at 2058
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1170
    Illegal PstI site found at 1257
    Illegal PstI site found at 1321
    Illegal PstI site found at 1693
    Illegal PstI site found at 1981
    Illegal PstI site found at 2058
    Illegal NgoMIV site found at 3816
  • 1000
    COMPATIBLE WITH RFC[1000]