Difference between revisions of "Part:BBa K3589201"

 
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<partinfo>BBa_K3589201 short</partinfo>
 
<partinfo>BBa_K3589201 short</partinfo>
  
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This composite part contains the PAR-promoter (<a href="https://parts.igem.org/Part:BBa_K3002027">BBa_K3002027</a>) in combination with the RPL23-Terminator (<a href="https://parts.igem.org/Part:BBa_K3002006">BBa_K3002006</a>), a 3xHA-tag (<a href="https://parts.igem.org/Part:BBa_K3002017">BBa_K3002017</a>) and the coding sequence of the mutant laccase from <I>Botrytis aclada</i> (<a href="https://parts.igem.org/Part:BBa_K3589108">BBa_K3589108</a>) plus the MoClo connectors for positions B2-B3 ( <a href="https://parts.igem.org/Part:BBa_K3002303">BBa_K3002303</a>), B4-B5 ( <a href="https://parts.igem.org/Part:BBa_K3002304">BBa_K3002304</a>) and B5-B6 (<a href="https://parts.igem.org/Part:BBa_K3002305">BBa_K3002305</a>).
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 17:04, 25 October 2020


Level 1 Mutant BaLac + HA (Phytobrick)

This composite part contains the PAR-promoter (BBa_K3002027) in combination with the RPL23-Terminator (BBa_K3002006), a 3xHA-tag (BBa_K3002017) and the coding sequence of the mutant laccase from Botrytis aclada (BBa_K3589108) plus the MoClo connectors for positions B2-B3 ( BBa_K3002303), B4-B5 ( BBa_K3002304) and B5-B6 (BBa_K3002305). Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1103
    Illegal PstI site found at 1190
    Illegal PstI site found at 1254
    Illegal PstI site found at 1626
    Illegal PstI site found at 1914
    Illegal PstI site found at 1991
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 249
    Illegal PstI site found at 1103
    Illegal PstI site found at 1190
    Illegal PstI site found at 1254
    Illegal PstI site found at 1626
    Illegal PstI site found at 1914
    Illegal PstI site found at 1991
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1550
    Illegal BglII site found at 2509
    Illegal BamHI site found at 1889
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1103
    Illegal PstI site found at 1190
    Illegal PstI site found at 1254
    Illegal PstI site found at 1626
    Illegal PstI site found at 1914
    Illegal PstI site found at 1991
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1103
    Illegal PstI site found at 1190
    Illegal PstI site found at 1254
    Illegal PstI site found at 1626
    Illegal PstI site found at 1914
    Illegal PstI site found at 1991
    Illegal NgoMIV site found at 3630
  • 1000
    COMPATIBLE WITH RFC[1000]