Difference between revisions of "Part:BBa K128003"

 
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<partinfo>BBa_K128003 short</partinfo>
 
<partinfo>BBa_K128003 short</partinfo>
  
 
This sequence codes for a short peptide, found to competitively inhibit binding of S.mutans to the tooth surface (CG et al.; Nature Biotechnol. 1999). S.mutans takes in sugars and secretes lactic acid, causing dental cavities, so introduction of this peptide into the mouth prevents cavities. This part uses the modified Silver BioBrick prefix and suffix to allow for protein construction.
 
This sequence codes for a short peptide, found to competitively inhibit binding of S.mutans to the tooth surface (CG et al.; Nature Biotechnol. 1999). S.mutans takes in sugars and secretes lactic acid, causing dental cavities, so introduction of this peptide into the mouth prevents cavities. This part uses the modified Silver BioBrick prefix and suffix to allow for protein construction.
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from MIT iGEM 2008: this research team has shown p1025 inhibiting binding to hydroxyapatite (synthetic teeth) through a "novel tooth binding assay".  The following graph shows the S. Mutans that are not bound to hydroxyapatite cell count.  When p1025 was present, there were more cells unbound in t1, meaning that fewer cells bound to the hydroxyapatite.
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For more about the "novel toothbinding assay": *[[http://2008.igem.org/Team:MIT/Tooth_binding_assay_protocol|Novel tooth binding assay]]
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[[Image:p1025.png| 300px| Variable plate layout]]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 18:49, 29 October 2008

p1025

This sequence codes for a short peptide, found to competitively inhibit binding of S.mutans to the tooth surface (CG et al.; Nature Biotechnol. 1999). S.mutans takes in sugars and secretes lactic acid, causing dental cavities, so introduction of this peptide into the mouth prevents cavities. This part uses the modified Silver BioBrick prefix and suffix to allow for protein construction.

from MIT iGEM 2008: this research team has shown p1025 inhibiting binding to hydroxyapatite (synthetic teeth) through a "novel tooth binding assay". The following graph shows the S. Mutans that are not bound to hydroxyapatite cell count. When p1025 was present, there were more cells unbound in t1, meaning that fewer cells bound to the hydroxyapatite.

For more about the "novel toothbinding assay": *Novel tooth binding assay

Variable plate layout

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 81
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 16
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 82
    Illegal PstI site found at 96
    Illegal NotI site found at 7
    Illegal NotI site found at 89
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 82
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 82
    Illegal PstI site found at 96
    Illegal NgoMIV site found at 42
  • 1000
    COMPATIBLE WITH RFC[1000]