Difference between revisions of "Part:BBa K3429001"

Revision as of 15:23, 25 October 2020

TasA matrix protein

Name: TasA matrix protein
Base pairs: 786 bp
Molecular Weight: 28.15 kDa
Origin: <li Bacillus subtilis </li>
The TasA protein (also known as the major biofilm matrix component) is essential for biofilm structures and polymerizes into long fibers attached to the B. subtilis cells. ^{[1] [2]}
We designed TasA fusion proteins with CotA, CuO and EreB to immobilize our degrading enzymes in the biofilm matrix. Protein secretion into the biofilm matrix is regulated by the Sec-dependent signal recognition partice (sec-SRP) pathway. ^[3]

References

[1] Branda, S.; Chu, F.; Kearns, D. (2006): A major protein component of the Bacillus subtilis biofilm matrix. In: Molecular microbiology 59 (4), S. 1229–1238, https://doi.org/10.1111/j.1365-2958.2005.05020.x

[2] Romero, D.; Aguilar, C.; Losick, R. (2010): Amyloid fibers provide structural integrity to Bacillus subtilis biofilms. In: Proceedings of the National Academy of Sciences of the United States of America 107 (5), S. 2230–2234, https://doi.org/10.1073/pnas.0910560107x

[3] Stöver, A.; Driks, A. (1999): Secretion, Localization, and Antibacterial Activity of TasA, a Bacillus subtilis Spore-Associated Protein. In: Journal of Bacteriology 181 (5), S. 1664–1672, https://doi.org/10.1128/JB.181.5.1664-1672.1999

Sequence and Features