Difference between revisions of "Part:BBa K3657022"
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You can generate analogical parts yourself as following: | You can generate analogical parts yourself as following: | ||
Revision as of 14:12, 25 October 2020
RNA Part of an RNA·DNA-DNA triple helix
!!!This part works in combination with the part BBa_K3657023.!!!
It was proved by Knucker et al. that the transcript of this sequence creates a triple helix with the DNA part (BBa_K3657023) in vitro.
More information available here: LINK
Usage and Biology
Part Design
You can generate analogical parts yourself as following:
- Generate a random DNA sequence containing adenine, cytosine and guanine. You sequence should be longer than 19 base pairs and preferably should not contain thymine.
- Use the following script to generate RNA: LINK. Corresponding bases in the script are the pairs that create strongest bonds according to Knucker et al.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]