Difference between revisions of "Part:BBa K3657022"

Revision as of 14:09, 25 October 2020

RNA Part of an RNA·DNA-DNA triple helix

!!!This part works in combination with the part BBa_K3657023 .!!!

It was proved by Knucker et al. that the transcript of this sequence creates a triple helix with the DNA part (BBa_K3657023) in vitro.

More information available here: LINK

You can generate analogical parts yourself as following:

Generate a random DNA sequence containing adenine, cytosine and guanine. You sequence should be longer than 19 base pairs and preferably should not contain thymine.
Use the following script to generate RNA: LINK. Corresponding bases in the script are the pairs that create strongest bonds according to Knucker et al.

Sequence and Features

Assembly Compatibility:

@@ Line 17: / Line 17: @@
 <ol>
     <li>Generate a random DNA sequence containing adenine, cytosine and guanine. You sequence should be longer than 19 base pairs and preferably should not contain thymine.</li>
-    <liUse the following script to generate RNA: LINK. Corresponding bases in the script are the pairs that create strongest bonds according to Knucker et al.</li>
+    <li>Use the following script to generate RNA: LINK. Corresponding bases in the script are the pairs that create strongest bonds according to Knucker et al.</li>
 </ol>
 </p>