Difference between revisions of "Part:BBa K3657022"
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<p>
 
You can generate analogical parts yourself as following:
 
You can generate analogical parts yourself as following:
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1. Generate a random DNA sequence containing adenine, cytosine and guanine. You sequence should be longer than 19 base pairs and preferably should not contain thymine.
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<ol>
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2. Use the following script to generate RNA: LINK. Corresponding bases in the script are the pairs that create strongest bonds according to Knucker et al.
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  <li>Generate a random DNA sequence containing adenine, cytosine and guanine. You sequence should be longer than 19 base pairs and preferably should not contain thymine.</li>
 +
  <liUse the following script to generate RNA: LINK. Corresponding bases in the script are the pairs that create strongest bonds according to Knucker et al.</li>
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</ol>
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</p>
  
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 14:09, 25 October 2020


RNA Part of an RNA·DNA-DNA triple helix

!!!This part works in combination with the part BBa_K3657023 .!!!

It was proved by Knucker et al. that the transcript of this sequence creates a triple helix with the DNA part (BBa_K3657023) in vitro.

More information available here: LINK

You can generate analogical parts yourself as following:

  1. Generate a random DNA sequence containing adenine, cytosine and guanine. You sequence should be longer than 19 base pairs and preferably should not contain thymine.
    2. <liUse the following script to generate RNA: LINK. Corresponding bases in the script are the pairs that create strongest bonds according to Knucker et al.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]