Difference between revisions of "Part:BBa K3629021"
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===Usage and Biology=== | ===Usage and Biology=== | ||
mCherry is a monomeric red fluorescent protein that is derived from DsRed of <i>Discosoma.</i> mCherry's optimal excitation wavelength is 586nm and its optimal emission wavelength is 610nm. mCherry could be used to tag components and proteins within the cell which can then be visualized using fluorescence spectroscopy and microscopy. | mCherry is a monomeric red fluorescent protein that is derived from DsRed of <i>Discosoma.</i> mCherry's optimal excitation wavelength is 586nm and its optimal emission wavelength is 610nm. mCherry could be used to tag components and proteins within the cell which can then be visualized using fluorescence spectroscopy and microscopy. | ||
+ | |||
+ | See [https://parts.igem.org/Part:BBa_K3629025 BBa_K3629025] for a functional high expression construct using this coding sequence. | ||
===Sequence and Features=== | ===Sequence and Features=== |
Revision as of 10:57, 25 October 2020
mCherry codon optimized for Yarrowia lipolytica
mCherry coding sequence codon-optimized for Yarrowia lipolytica.
Usage and Biology
mCherry is a monomeric red fluorescent protein that is derived from DsRed of Discosoma. mCherry's optimal excitation wavelength is 586nm and its optimal emission wavelength is 610nm. mCherry could be used to tag components and proteins within the cell which can then be visualized using fluorescence spectroscopy and microscopy.
See BBa_K3629025 for a functional high expression construct using this coding sequence.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]